STK32C Antibody / Serine/threonine-protein kinase 32C (FY12510)

Flow Cytometry analysis of Hela cells using anti-STK32C antibody. Overlay histogram showing Hela cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STK32C antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of STK32C using anti-STK32C antibody. Lane 1: human whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK32C antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. STK32C (~55 kDa predicted) was detected as a major band at ~60 kDa, consistent with the known canonical isoform migrating slightly above its calculated molecular weight, and a minor band at ~43 kDa, which may represent a shorter splice variant or proteolytic fragment of STK32C.