STK25 Antibody / Serine/threonine-protein kinase 25 Antibody (FY13586)

STK25 Antibody by FACS in U251 cells. Flow cytometry analysis using Serine/threonine-protein kinase 25 Antibody shows a rightward shift of the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unstained blank (red). U251 cells were fixed with 4% paraformaldehyde and permeabilized for intracellular staining. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by a fluorescent anti-rabbit IgG secondary antibody. The histogram demonstrates detectable antibody-associated fluorescence under these assay conditions.

STK25 Antibody by WB in human HepG2 and U251 cells. Western blot analysis using Serine/threonine-protein kinase 25 Antibody detects a prominent band near 48 kDa, compared with the calculated molecular weight of approximately 48 kDa. Lane 1 contains HepG2 whole-cell lysate and lane 2 contains U251 whole-cell lysate. Reduced lysates containing 30 ug total protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection employed an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate.