STING1 Antibody / cGAS-STING Pathway Antibody (FY13022)

STING1 Antibody in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with STING1 Antibody at 2 ug/ml. HIER was performed using EDTA buffer, pH 8.0, followed by blocking with 10% goat serum and overnight primary antibody incubation at 4oC. Detection used an HRP-conjugated secondary antibody with DAB substrate. Strong brown immunoreactivity is visible predominantly within the cytoplasm of malignant epithelial cells, while stromal and inflammatory regions show comparatively limited staining. The result demonstrates detection of STING1/TMEM173, a central component of the cGAS-STING pathway, in human lung cancer tissue.

STING1 Antibody Detects STING in Human and Mouse Samples WB. Western blot analysis using STING1 Antibody at 0.5 ug/ml. Lane 1: human Jurkat whole cell lysate; lane 2: mouse spleen tissue lysate; lane 3: mouse thymus tissue lysate; lane 4: mouse MH-S cell lysate. A prominent band is detected at approximately 37 kDa across all four samples, slightly below the predicted molecular weight of approximately 42 kDa. This migration behavior has been reported for the multipass transmembrane STING1 protein and is consistent across the human and mouse samples shown, supporting detection of the cGAS-STING pathway component STING1/TMEM173.

STING1 Antibody in SiHa Cervical Cancer Cells IF. Immunofluorescence staining of human SiHa cervical cancer cells with STING1 Antibody at 5 ug/ml (green) and a Beta Tubulin antibody (red). Cells were blocked with 10% goat serum and incubated with both primary antibodies overnight at 4oC, followed by fluorescent secondary antibody detection. STING1 displays prominent intracellular green fluorescence with a broadly cytoplasmic distribution, while Beta Tubulin highlights the cytoskeletal network in red. The merged image demonstrates detection and intracellular localization of STING1/TMEM173, an essential signaling protein of the cGAS-STING pathway.

STING1 Antibody in HepG2 Liver Cancer Cells FACS. Flow cytometry analysis of PFA-fixed human HepG2 liver cancer cells using STING1 Antibody at 1 ug/million cells. Cells were blocked with 10% normal goat serum and incubated with primary antibody for 30 min at 20oC, followed by DyLight 488-conjugated secondary antibody. STING1-stained cells (blue) show a strong rightward fluorescence shift relative to the isotype control (green) and blank control (red), with clear separation among the populations. The result demonstrates detection of intracellular STING1/TMEM173 in HepG2 cells.