STING Antibody for FACS / STING Flow Cytometry Antibody (R32276)

IHC analysis of STING/TMEM173 Antibody. STING expression was examined in a paraffin-embedded section of human lung cancer tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), sections were blocked with goat serum and incubated with a rabbit anti-STING/TMEM173 antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. STING staining is observed predominantly in tumor epithelial cells with cytoplasmic and membranous localization patterns, while surrounding stromal areas show lower background signal.

Western blot analysis of STING/TMEM173 Antibody. Proteins were resolved by 10% SDS-PAGE and transferred to a nitrocellulose membrane prior to immunodetection. Lane 1: human 293T whole cell lysates; Lane 2: human Jurkat whole cell lysates; Lane 3: mouse spleen tissue lysates; Lane 4: mouse thymus tissue lysates; Lane 5: mouse MH-S whole cell lysates. A prominent band corresponding to TMEM173 was detected at approximately 35 kDa across multiple samples. Although the predicted molecular weight of STING is approximately 42 kDa based on amino acid sequence, STING is well documented to migrate at a lower apparent molecular weight on SDS-PAGE, likely due to its multi-pass transmembrane architecture, hydrophobic regions, and detergent-dependent electrophoretic behavior. Lower apparent migration and subtle band heterogeneity have been reported for STING in both human and mouse tissues, and the observed banding pattern is consistent with published studies of endogenous TMEM173 expression.

STING Antibody for FACS flow cytometry analysis of human cells. Flow cytometric analysis of fixed human HepG2 cells using STING Antibody for FACS at 1 ug per million cells demonstrates intracellular detection of Stimulator of interferon genes protein / STING1 (TMEM173). Cells were blocked with goat sera prior to antibody staining and analyzed following fluorescent secondary detection. The blue histogram represents STING antibody staining and shows a clear rightward fluorescence shift relative to the green isotype control, confirming specific detection of intracellular STING protein. The red histogram represents unstained cells and defines baseline autofluorescence levels in the HepG2 population.