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Home >> Antibodies >> STEA2 Antibody

STEA2 Antibody (F54829)

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  Catalog No Formulation Size Price (USD)  
Image F54829-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54829-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms).
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Western blot testing of human HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide, human HepG2 cell lysate in the 3) absence and 4) presence of immunizing peptide, and mouse spleen tissue lysate in the 5) absence and 6) presence of immunizing peptide with STEA2 antibody. Predicted molecular weight: 48-56 kDa (3 isoforms).
IHC testing of FFPE human prostate carcinoma tissue with STEA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with STEA2 antibody; Blue=isotype control, Green= STEA2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt Q8NFT2
Localization Cytoplasmic
Applications Western blot : 1:500-1:1000
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This STEA2 antibody is available for research use only.
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Description

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Application Notes

The stated application concentrations are suggested starting points. Titration of the STEA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 229-258 from the human protein was used as the immunogen for the STEA2 antibody.

Storage

Aliquot the STEA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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