STAT6 Antibody [clone STAT6/7774R] (V4302)

Immunohistochemistry analysis of STAT6 antibody in human cerebellum tissue (clone STAT6/7774R). FFPE human cerebellum sections demonstrate HRP-DAB brown staining predominantly in the cytoplasm and nuclei of scattered cells within the cerebellar cortex and white matter regions. The staining pattern is consistent with Signal transducer and activator of transcription 6 localization, reflecting its cytoplasmic presence with potential nuclear translocation upon activation. Background neuropil shows minimal non-specific staining. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, demonstrating absence of specific brown staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Immunohistochemistry analysis of STAT6 antibody in human liposarcoma tissue (clone STAT6/7774R). FFPE human liposarcoma sections demonstrate HRP-DAB brown nuclear staining in scattered tumor cells within a fibrous stromal background. The predominant nuclear localization is consistent with activated Signal transducer and activator of transcription 6, which translocates to the nucleus upon phosphorylation. Cytoplasmic staining is minimal, and surrounding stromal elements show limited background signal. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.