STAT1 Antibody / JAK-STAT Pathway Transcription Factor (R32722)

STAT1 Antibody Multi-Cell Line WB. Western blot analysis of human HeLa, PC-3, Caco-2, A549, K562, and Raji cell lysates using STAT1 antibody demonstrates prominent immunoreactive bands at approximately 91 and 84 kDa, corresponding to the alpha and beta isoforms of Signal Transducer and Activator of Transcription 1 (STAT1). STAT1 is a key transcription factor within the JAK-STAT signaling pathway that mediates cellular responses to interferons, cytokines, and growth factors. The broad detection of STAT1 across multiple epithelial and hematopoietic cell lines is consistent with its central role in signal transduction, immune regulation, transcriptional control, and cellular adaptation to extracellular signaling stimuli. Western blot was performed using 0.5 ug/ml primary antibody. Predicted molecular weights: ~91 kDa (STAT1 alpha) and ~84 kDa (STAT1 beta).

STAT1 Antibody Thymus WB. Western blot analysis of rat thymus and mouse thymus tissue lysates using STAT1 antibody demonstrates prominent immunoreactive bands at approximately 91 and 84 kDa, corresponding to the alpha and beta isoforms of Signal Transducer and Activator of Transcription 1 (STAT1). STAT1 is a central transcription factor within the JAK-STAT signaling pathway that mediates cellular responses to interferons, cytokines, and immune regulatory signals. The strong expression observed in thymic tissues is consistent with the important role of STAT1 in immune system development, cytokine signaling, and transcriptional regulation within lymphoid organs. Western blot was performed using 0.5 ug/ml primary antibody. Predicted molecular weights: ~91 kDa (STAT1 alpha) and ~84 kDa (STAT1 beta).

STAT1 Antibody Thymus WB. Western blot analysis of rat thymus and mouse thymus tissue lysates using STAT1 antibody demonstrates prominent immunoreactive bands at approximately 91 kDa and 84 kDa, consistent with the alpha and beta isoforms of Signal Transducer and Activator of Transcription 1 (STAT1). STAT1 is a central transcription factor within the JAK-STAT signaling pathway that mediates cellular responses to interferons, cytokines, and immune regulatory signals. The strong expression observed in thymic tissues is consistent with the important role of STAT1 in immune system development, cytokine signaling, and transcriptional regulation within lymphoid organs. Western blot was performed using 0.5 ug/ml primary antibody. Predicted molecular weights: approximately 91 kDa for the alpha isoform and 84 kDa for the beta isoform.

STAT1 Antibody Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue using STAT1 antibody demonstrates strong cytoplasmic and nuclear staining within neoplastic epithelial cell populations, consistent with expression of Signal Transducer and Activator of Transcription 1 (STAT1). STAT1 is a key transcription factor within the JAK-STAT signaling pathway that mediates cellular responses to interferons, cytokines, and other extracellular signaling molecules. The observed staining pattern is consistent with the role of STAT1 in regulating transcriptional programs involved in immune responses, cellular signaling, differentiation, and tumor-associated signaling networks. These findings support the utility of STAT1 antibody for studies of signal transduction, immune regulation, and transcription factor expression in human tissues. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

STAT1 Antibody U-2 OS IF/ICC. Immunofluorescent staining of FFPE human U-2 OS cells using STAT1 antibody demonstrates prominent green fluorescence within both the nucleus and cytoplasm, consistent with the known subcellular localization and signaling function of Signal Transducer and Activator of Transcription 1 (STAT1). STAT1 is a key transcription factor within the JAK-STAT signaling pathway that undergoes activation-dependent nuclear translocation to regulate expression of interferon-responsive and cytokine-regulated genes. The observed staining pattern is consistent with the role of STAT1 in transcriptional regulation, immune signaling, and cellular responses to extracellular stimuli. Nuclei are visualized with DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

STAT1 Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells using STAT1 antibody demonstrates strong green fluorescence predominantly within the nucleus, consistent with the role of Signal Transducer and Activator of Transcription 1 (STAT1) as a transcription factor that translocates to the nucleus following activation. STAT1 is a central mediator of JAK-STAT signaling and regulates expression of genes involved in interferon responses, cytokine signaling, immune regulation, and cellular adaptation to extracellular stimuli. The observed nuclear staining pattern is consistent with transcriptionally active STAT1 participating in signal-dependent gene regulation. Nuclei are visualized with DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

STAT1 Antibody A431 Flow Cytometry. Flow cytometric analysis of human A431 cells stained with STAT1 antibody demonstrates a clear rightward shift of the blue histogram (STAT1 antibody) relative to the green histogram (isotype control) and red histogram (cells alone), consistent with expression of Signal Transducer and Activator of Transcription 1 (STAT1). STAT1 is a key transcription factor within the JAK-STAT signaling pathway that mediates cellular responses to interferons, cytokines, and growth factors. The distinct separation between the STAT1-stained population and controls supports specific detection of STAT1 and demonstrates the utility of this antibody for flow cytometric studies of signal transduction, immune regulation, and cytokine-responsive cellular pathways. Cells were blocked with goat serum and stained using 1 ug antibody per 10^6 cells.