STAM2 Antibody / Signal transducing adaptor molecule 2 (FY13090)

STAM2 Antibody U2OS Cell IF. Immunofluorescent staining of fixed and permeabilized U2OS cells with STAM2 Antibody / Signal Transducing Adaptor Molecule 2 Antibody (red) and a Beta Tubulin antibody (green). Cells underwent enzyme antigen retrieval for 15 minutes, were blocked with 10% goat serum, and incubated overnight at 4 degrees C with rabbit anti-STAM2 antibody (5 ug/ml) and mouse anti-Beta Tubulin antibody. Cy3- and DyLight 488-conjugated secondary antibodies were used at a 1:500 dilution. STAM2 displays punctate cytoplasmic staining with strong perinuclear localization, consistent with its association with early and sorting endosomes. This STAM2 Antibody, also called Signal Transducing Adaptor Molecule 2 Antibody, is suitable for studies of endosomal trafficking and vesicle transport.

STAM2 Antibody Human and Rodent WB. Western blot analysis of human HepG2 whole cell lysate (lane 1), rat liver (lane 2), rat brain (lane 3), and mouse brain (lane 4) using STAM2 Antibody / Signal Transducing Adaptor Molecule 2 Antibody at 0.5 ug/ml. A prominent band is detected at approximately 65-70 kDa, consistent with the known phosphorylation-dependent mobility shift of STAM2 from its predicted molecular weight of approximately 58-60 kDa. Brain tissue lysates show a characteristic doublet, likely representing different phosphorylation states and/or the STAM2A and STAM2B isoforms. This STAM2 Antibody, also called Signal Transducing Adaptor Molecule 2 Antibody, is suitable for studies of endosomal trafficking and intracellular signaling.

STAM2 Antibody Human, Rat and Mouse WB. Western blot analysis of human HeLa (lane 1), 293T (lane 2), Jurkat (lane 3), and HepG2 (lane 4) whole cell lysates, rat brain (lane 5) and testis (lane 6), and mouse testis (lane 7) using STAM2 Antibody / Signal Transducing Adaptor Molecule 2 Antibody at 0.5 ug/ml. A specific band is detected at approximately 65-70 kDa across multiple species and sample types, consistent with the known phosphorylation-dependent mobility shift from the predicted molecular weight of approximately 58 kDa. This STAM2 Antibody, also called Signal Transducing Adaptor Molecule 2 Antibody, is suitable for studies of endosomal trafficking and intracellular signaling.

STAM2 Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using STAM2 Antibody / Signal Transducing Adaptor Molecule 2 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer before incubation with rabbit anti-STAM2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. STAM2 immunoreactivity is predominantly cytoplasmic with moderate to strong staining in malignant epithelial cells, consistent with the protein's role in endosomal sorting and intracellular vesicle trafficking. This STAM2 Antibody, also called Signal Transducing Adaptor Molecule 2 Antibody, is suitable for investigating STAM2 expression in human tissues.

STAM2 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using STAM2 Antibody / Signal Transducing Adaptor Molecule 2 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer before incubation with rabbit anti-STAM2 antibody at 2 ug/ml. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. STAM2 shows moderate to strong predominantly cytoplasmic staining in tumor cells, consistent with its role in endosomal sorting and intracellular vesicle trafficking. This STAM2 Antibody, also called Signal Transducing Adaptor Molecule 2 Antibody, is suitable for investigating STAM2 expression in human tissues.

STAM2 Antibody A549 Cell IF. Immunofluorescent staining of fixed and permeabilized A549 cells with STAM2 Antibody / Signal Transducing Adaptor Molecule 2 Antibody (green) and an Alpha Tubulin antibody (red). Cells underwent enzyme antigen retrieval for 15 minutes, were blocked with 10% goat serum, and incubated overnight at 4 degrees C with rabbit anti-STAM2 antibody at 5 ug/ml and mouse anti-Alpha Tubulin antibody. Fluoro488- and Cy3-conjugated secondary antibodies were used at a 1:500 dilution. STAM2 exhibits predominantly cytoplasmic staining with a fine punctate distribution, consistent with its localization to endosomal compartments. This STAM2 Antibody, also called Signal Transducing Adaptor Molecule 2 Antibody, is suitable for studies of endosomal trafficking and intracellular vesicle dynamics.