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Home >> Antibodies >> SSEA-1 Antibody

SSEA-1 Antibody [clone SPM490] (V2523)

  Catalog No Formulation Size Price (USD)  
Image V2523-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2523-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2523SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human U937 cells with Calponin antibody (clone SPM490, green) and Reddot nuclear stain (red).
IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with SSEA-1 antibody (clone SPM490).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name SPM490
Purity PEG precipitation
UniProt P22083
Localization Cell surface and granular paranuclear
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This SSEA-1 antibody is available for research use only.
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Description

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Application Notes

Optimal dilution of the SSEA-1 antibody should be determined by the researcher.

Immunogen

Myelomonocytic leukemia cells were used as the immunogen for the SSEA-1 antibody.

Storage

Store the SSEA-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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