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Home >> Antibodies >> SRF Antibody / Serum Response Factor

SRF Antibody / Serum Response Factor [clone PCRP-SRF-1F1] (V9726)

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  Catalog No Formulation Size Price (USD)  
Image V9726-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9726-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9726SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SRF antibody (clone PCRP-SRF-1F1). These results demonstrate the foremost specificity of the PCRP-SRF-1F1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SRF antibody (clone PCRP-SRF-1F1). These results demonstrate the foremost specificity of the PCRP-SRF-1F1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with SRF antibody (blue, clone PCRP-SRF-1F1) and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using SRF antibody (green, clone PCRP-SRF-1F1) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free SRF antibody (clone PCRP-SRF-1F1) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name PCRP-SRF-1F1
Purity Protein A/G affinity
UniProt P11831
Localization Nucleus
Applications ELISA (order BSA-free format for coating) :
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Limitations This SRF antibody is available for research use only.
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Description

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.

Application Notes

Optimal dilution of the SRF antibody should be determined by the researcher.

Immunogen

Recombinant full-length human SRF protein was used as the immunogen for the SRF antibody.

Storage

Aliquot the SRF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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