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Home >> Antibodies >> SREBP2 Antibody / SREBF2

SREBP2 Antibody / SREBF2 (RQ6804)

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  Catalog No Formulation Size Price (USD)  
Image RQ6804 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human MCF7 cells with SREBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with SREBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human ThP-1, 2) human Jurkat, 3) human Raji, 4) human HL60, 5) rat stomach, 6) rat testis, 7) mouse stomach and 8) rat testis tissue lysate with SREBP2 antibody. Predicted molecular weight ~124 kDa but may be observed at higher molecular weights due to glycosylation. The active form of this protein can be observed at 50-68 kDa.
Flow cytometry testing of human K562 cells with SREBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SREBP2 antibody.
Flow cytometry testing of rat RH35 cells with SREBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SREBP2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q12772
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SREBP2 antibody is available for research use only.
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Description

Sterol regulatory element-binding protein 2 (SREBP-2) also known as sterol regulatory element binding transcription factor 2 (SREBF2) is a protein that in humans is encoded by the SREBF2 gene. This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the SREBP2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R371-L409) was used as the immunogen for the SREBP2 antibody.

Storage

After reconstitution, the SREBP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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