SRBI Antibody / HDL Receptor Antibody (FY13489)

SRBI Antibody HeLa Cell IF. Immunofluorescence staining of HeLa cells with SRBI Antibody demonstrated predominantly cytoplasmic and plasma membrane-associated staining (red), while nuclei were counterstained with DAPI (blue). Cells underwent enzyme-mediated antigen retrieval before incubation with the primary antibody and a Cy3-conjugated secondary antibody. The staining pattern is consistent with the localization of SRBI as an HDL Receptor Antibody target involved in cholesterol uptake and lipoprotein transport, supporting its use for immunofluorescence studies of lipid metabolism and membrane receptor biology.

SRBI Antibody Human and Mouse WB. Western blot analysis detected SRBI in human hepatocellular carcinoma tissue (lane 1), human HepG2 cells (lane 2), human MCF-7 cells (lane 3) and mouse liver tissue (lane 4). A prominent immunoreactive band was observed at approximately 85 kDa, which is higher than the predicted molecular weight of about 61 kDa and is consistent with the known glycosylation of this membrane receptor. Additional higher molecular weight bands may represent different glycosylated forms. This SRBI Antibody, an HDL Receptor Antibody, is suitable for detecting SRBI across human and mouse samples in studies of cholesterol transport and lipoprotein biology.

SRBI Antibody HepG2 Cell FACS. Flow cytometry analysis of fixed HepG2 cells demonstrated a pronounced rightward fluorescence shift with SRBI Antibody (blue) compared with the isotype control (green) and unstained cells (red), indicating specific detection of endogenous SRBI expression. Cells were stained with 1 ug antibody per 1×10^6 cells followed by a Fluoro488-conjugated secondary antibody. This SRBI Antibody, an HDL Receptor Antibody, is suitable for flow cytometric analysis of cell surface receptor expression in studies of cholesterol transport, lipoprotein metabolism and cardiovascular biology.