SQSTM1 Antibody / p62 [clone 3H11] (RQ5514)

Immunofluorescent staining of FFPE human A431 cells with SQSTM1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer with SQSTM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer with SQSTM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human colon cancer with SQSTM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Western blot testing of human 1) CCRF-CEM, 2) HepG2, 3) HT1080, 4) SW620 lysate with SQSTM1 antibody. Routinely observed at ~62 kDa.

Western blot testing of 1) rat brain, 2) rat liver, 3) rat PC-12, 4) rat RH35, 5) mouse brain, 6) mouse liver, 7) mouse NIH3T3, 8) mouse RAW246.7 and 9) mouse Neuro-2a lysate with SQSTM1 antibody. Routinely observed at ~62 kDa.

IHC staining of FFPE mouse intestine with SQSTM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestine with SQSTM1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Flow cytometry testing of human PC-3 cells with SQSTM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SQSTM1 antibody.

Flow cytometry testing of human A549 cells with SQSTM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SQSTM1 antibody.

Immunofluorescent staining of FFPE human MCF7 cells with SQSTM1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.