SPTAN1 Antibody Protein Microarray Validated / Alpha II Spectrin [clone SPTAN1/3505] (V9584)

Immunohistochemistry of SPTAN1 antibody in human lung tissue. FFPE human lung shows cytoplasmic HRP-DAB brown staining along alveolar septa and within pneumocytes, consistent with Spectrin alpha chain, non-erythrocytic 1 localization along the submembranous cytoskeleton. Clone SPTAN1/3505 was used for detection and is Protein Microarray Validated. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

Immunohistochemistry of SPTAN1 antibody in human placental tissue. FFPE placenta shows cytoplasmic HRP-DAB brown staining within trophoblastic cells outlining chorionic villi, consistent with Spectrin alpha chain, non-erythrocytic 1 localization along the submembranous cytoskeleton. Clone SPTAN1/3505 was used for detection and is Protein Microarray Validated. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free SPTAN1 antibody (clone SPTAN1/3505) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPTAN1 antibody (clone SPTAN1/3505). These results demonstrate the foremost specificity of the SPTAN1/3505 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.