SPTAN1 Antibody | Cytoskeletal Spectrin Marker

	Catalog No	Formulation	Size	Price (USD)
	V9584-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V9584-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V9584SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

SPTAN1 Antibody Human Placenta IHC. Immunohistochemistry of FFPE human placental tissue using SPTAN1 Antibody / Cytoskeletal Spectrin Marker, clone SPTAN1/3505, shows cytoplasmic HRP-DAB brown staining within trophoblastic cells outlining chorionic villi, consistent with SPTAN1 localization along the submembranous cytoskeleton. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining. This Protein Microarray Validated SPTAN1 Antibody demonstrates detection of the cytoskeletal spectrin marker in human placental tissue.

SDS-PAGE analysis of purified, BSA-free SPTAN1 antibody (clone SPTAN1/3505) as confirmation of integrity and purity.

SPTAN1 Antibody Protein Microarray Specificity Validation. Protein microarray analysis of SPTAN1 Antibody / Cytoskeletal Spectrin Marker, clone SPTAN1/3505, was performed using a HuProt(TM) microarray containing more than 19,000 full-length human proteins. The Z-score represents antibody signal strength in standard deviations above the mean signal across the array. The S-score represents the difference between the Z-scores of the intended target and the next highest-ranking signal, providing a measure of relative target specificity. The results demonstrate strong and highly selective recognition of SPTAN1 by clone SPTAN1/3505.

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SPTAN1 Antibody Human Lung IHC. Immunohistochemistry of FFPE human lung tissue using SPTAN1 Antibody / Cytoskeletal Spectrin Marker, clone SPTAN1/3505, shows cytoplasmic HRP-DAB brown staining along alveolar septa and within pneumocytes, consistent with SPTAN1 localization along the submembranous cytoskeleton. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining. This Protein Microarray Validated SPTAN1 Antibody demonstrates detection of the cytoskeletal spectrin marker in human lung tissue.

SPTAN1 Antibody Human Placenta IHC. Immunohistochemistry of FFPE human placental tissue using SPTAN1 Antibody / Cytoskeletal Spectrin Marker, clone SPTAN1/3505, shows cytoplasmic HRP-DAB brown staining within trophoblastic cells outlining chorionic villi, consistent with SPTAN1 localization along the submembranous cytoskeleton. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining. This Protein Microarray Validated SPTAN1 Antibody demonstrates detection of the cytoskeletal spectrin marker in human placental tissue.

SDS-PAGE analysis of purified, BSA-free SPTAN1 antibody (clone SPTAN1/3505) as confirmation of integrity and purity.

SPTAN1 Antibody Protein Microarray Specificity Validation. Protein microarray analysis of SPTAN1 Antibody / Cytoskeletal Spectrin Marker, clone SPTAN1/3505, was performed using a HuProt(TM) microarray containing more than 19,000 full-length human proteins. The Z-score represents antibody signal strength in standard deviations above the mean signal across the array. The S-score represents the difference between the Z-scores of the intended target and the next highest-ranking signal, providing a measure of relative target specificity. The results demonstrate strong and highly selective recognition of SPTAN1 by clone SPTAN1/3505.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	SPTAN1/3505
Purity	Protein A/G affinity
UniProt	Q13813
Localization	Cytoplasm
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations	This SPTAN1 Antibody / Cytoskeletal Spectrin Marker is available for research use only.
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Description

SPTAN1 Antibody / Cytoskeletal Spectrin Marker recognizes spectrin alpha chain, non-erythrocytic 1, a large cytoskeletal scaffolding protein encoded by the SPTAN1 gene. SPTAN1, also widely known as alpha II spectrin and alpha fodrin, is a major component of the spectrin-based membrane cytoskeleton in non-erythroid cells. It associates with beta spectrins and actin to form a flexible structural network beneath the plasma membrane, helping maintain cell shape, mechanical stability and the organization of membrane-associated proteins.

SPTAN1 is broadly expressed in human tissues and contributes to cytoskeletal architecture in epithelial, neuronal and other cell types. Its widespread distribution is consistent with its fundamental role in maintaining cellular structure rather than serving as a marker restricted to a single tissue or lineage. Spectrin complexes help connect membrane proteins to the actin cytoskeleton and participate in the spatial organization of ion channels, adhesion molecules and signaling proteins. These functions make SPTAN1 an important cytoskeletal spectrin marker for studies of membrane organization, cell morphology and structural integrity.

SPTAN1 has particularly important functions in the nervous system, where spectrin-based cytoskeletal networks contribute to axonal stability, dendritic organization and neuronal membrane architecture. Pathogenic variants in SPTAN1 have been associated with a range of neurodevelopmental and neurological disorders, including developmental and epileptic encephalopathies. Beyond neuronal biology, altered spectrin organization has also been investigated in cancer, cell migration and other processes involving changes in cytoskeletal structure.

Alpha fodrin is also well known for undergoing proteolytic cleavage during apoptosis and cellular injury. Calpains and caspases can generate characteristic SPTAN1 breakdown products, making spectrin proteolysis useful in research into apoptosis, neuronal injury, neurodegeneration and cytoskeletal damage. This relationship between structural integrity and regulated proteolysis gives SPTAN1 relevance to both basic cell biology and disease-focused research.

Clone SPTAN1/3505 is a mouse monoclonal antibody developed against human SPTAN1. Protein microarray validation provides an additional assessment of target specificity against a broad collection of full-length human proteins. In HuProt analysis, the Z-score represents signal strength in standard deviations above the mean signal across the array, while the S-score reflects the difference between the intended target and the next highest-ranking signal, providing a measure of relative target specificity. An SPTAN1 Antibody can support research into spectrin cytoskeletal organization, membrane stability, apoptosis-associated proteolysis and the structural biology of neuronal and non-neuronal cells.

SPTAN1 is a key component of the neuronal cytoskeleton, making it relevant to research using Neuroscience Antibodies to study neuronal structure, function and neurological disease.

Application Notes

Optimal dilution of the SPTAN1 Antibody / Cytoskeletal Spectrin Marker should be determined by the researcher.

Immunogen

A portion of amino acids 2351-2475 was used as the immunogen for the SPTAN1 antibody.

Storage

Aliquot the SPTAN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

SPTAN1 antibody, Spectrin alpha chain non-erythrocytic 1 antibody, Alpha II spectrin antibody, Alpha fodrin antibody, NEAS antibody

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SPTAN1 Antibody / Cytoskeletal Spectrin Marker [clone SPTAN1/3505] (V9584)

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