SPTAN1 Antibody for IHC / Alpha II Spectrin IHC Antibody [clone SPTAN1/3351] (V8095)

SPTAN1 Antibody for IHC staining of FFPE human colon tissue. HRP-DAB brown cytoplasmic staining is observed in epithelial and stromal cells, consistent with the submembranous cytoskeletal localization of Spectrin alpha chain, non-erythrocytic 1 (Alpha II Spectrin / SPTAN1). The rabbit monoclonal antibody clone SPTAN1/3351 was used for detection. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunohistochemistry of SPTAN1 antibody in human colon tissue. FFPE human colon shows cytoplasmic HRP-DAB brown staining in epithelial and stromal cells, consistent with Spectrin alpha chain, non-erythrocytic 1 localization along the submembranous cytoskeleton. Clone SPTAN1/3351 was used for detection. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPTAN1 antibody (clone SPTAN1/3351). These results demonstrate the foremost specificity of the SPTAN1/3351 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free SPTAN1 antibody as confirmation of integrity and purity.