Spectrin beta III Antibody / SPTBN2 [clone SPTBN2/1583] (V3395)

Immunofluorescent staining of MeOH fixed human HeLa cells with Spectrin beta III antibody (clone SPTBN2/1583, green) and Reddot nuclear stain (red).

IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1583). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human PANC-1 cell lysate with Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

Western blot testing of human HeLa cell lysate with Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

Flow cytometry staining of human HeLa cells with Spectrin beta III antibody; Red=isotype control, Blue= Spectrin beta III antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Spectrin beta III antibody (clone SPTBN2/1583). These results demonstrate the foremost specificity of the SPTBN2/1583 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1583). Confirmation of Integrity and Purity of the Antibody.