SPARC Antibody / Secreted protein acidic and rich in cysteine / Osteonectin [clone OSTN/3761] (V5796)

Immunohistochemistry analysis of SPARC antibody in human tonsil tissue (clone OSTN/3761). FFPE human tonsil sections show HRP-DAB brown cytoplasmic and extracellular stromal staining in scattered stromal cells and matrix-rich areas, consistent with Secreted protein acidic and rich in cysteine expression in the extracellular matrix. Surrounding lymphoid cells are largely negative, providing contrast against the positive stromal compartment. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

Western blot testing of human A375 cell lysate with SPARC antibody (clone OSTN/3761). Expected molecular weight: 35-43 kDa depending on glycosylation level.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPARC antibody (clone OSTN/3761). These results demonstrate the foremost specificity of the OSTN/3761 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.