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Home >> Antibodies >> SPARC Antibody

SPARC Antibody (R32676)

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  Catalog No Formulation Size Price (USD)  
Image R32676 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) rat testis, 3) mouse brain, 4) mouse testis, 5) human U-2 OS and 6) human HepG2 lysate with SPARC antibody at 0.5ug/ml. Predicted molecular weight ~35 kDa, routinely observed at ~43 and 50 kDa.
Western blot testing of 1) rat brain, 2) rat testis, 3) mouse brain, 4) mouse testis, 5) human U-2 OS and 6) human HepG2 lysate with SPARC antibody at 0.5ug/ml. Predicted molecular weight ~35 kDa, routinely observed at ~43 and 50 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P09486
Applications Western Blot : 0.5-1ug/ml
Limitations This SPARC antibody is available for research use only.
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Description

SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.

Application Notes

Optimal dilution of the SPARC antibody should be determined by the researcher.

Immunogen

Amino acids E107-I303 from the human protein were used as the immunogen for the SPARC antibody.

Storage

After reconstitution, the SPARC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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