SP100 Antibody [clone PCRP-SP100-1B9] (V8994)

FACS staining of PFA-fixed human HeLa cells with SP100 antibody (blue, clone PCRP-SP100-1B9), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free SP100 antibody (PCRP-SP100-1B9) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells using SP100 antibody (green, clone PCRP-SP100-1B9) and phalloidin (red).

IHC staining of FFPE human colon tissue with SP100 antibody (clone PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with SP100 antibody (clone PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SP100 antibody (clone PCRP-SP100-1B9). These results demonstrate the foremost specificity of the PCRP-SP100-1B9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.