SOX9 Antibody for WB / SOX9 Western Blot Antibody [clone SOX9/2398] (V7459)

SOX9 Antibody Human HepG2 WB. Western blot analysis of human HepG2 hepatocellular carcinoma cell lysate using SOX9 Antibody for WB. Mouse monoclonal antibody clone SOX9/2398 detects a band at approximately 48-50 kDa, slightly lower than the predicted molecular weight of SOX9 (56-65 kDa). SOX9 is a nuclear transcription factor involved in differentiation and cancer-associated signaling, and its detection in HepG2 cells supports expression in hepatic tumor models. The lower apparent molecular weight may reflect protein processing, isoform variation, or differences in electrophoretic migration.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX9/Transcription factor SOX-9 antibody. These results demonstrate the foremost specificity of the SOX9/2398 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free SOX9/Transcription factor SOX-9 antibody as confirmation of integrity and purity.

Immunohistochemistry analysis of SOX9 / Transcription factor SOX-9 antibody in human pancreas tissue. FFPE human pancreas sections show strong HRP-DAB brown nuclear staining in ductal epithelial cells and scattered epithelial cell populations, consistent with nuclear localization of SOX-9 as a transcription factor. Surrounding acinar cells demonstrate minimal background staining, while stromal elements are largely negative. The staining pattern highlights SOX-9 expression within pancreatic ductal structures and progenitor-associated compartments. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by boiling for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

IHC staining of FFPE human pancreas with SOX9/Transcription factor SOX-9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.