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Home >> Antibodies >> SOX9 Antibody / SRY-box transcription factor 9

SOX9 Antibody / SRY-box transcription factor 9 [clone SOX9/2387] (V8917)

  Catalog No Formulation Size Price (USD)  
Image V8917-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V8917-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V8917SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated
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Western blot testing of human PC3 cell lysate using SOX9 antibody (clone SOX9/2387). Predicted molecular weight: 56-65 kDa.
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric carcinoma tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SOX9 antibody (clone SOX9/2387) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX9 antibody (clone SOX9/2387). These results demonstrate the foremost specificity of the SOX9/2387 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SOX9/2387
Purity Protein A/G affinity
UniProt P48436
Localization Nucleus
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This SOX9 antibody is available for research use only.
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Description

SOX9 Antibody recognizes SRY-box transcription factor 9, encoded by the SOX9 gene and commonly referred to as SRY-box 9. SOX9 is a nuclear transcription factor belonging to the SRY-related HMG-box family and functions as a master regulator of lineage specification, differentiation, and developmental patterning. SOX9 Antibody detects a DNA-binding protein that localizes to the nucleus and controls gene expression programs essential for cartilage formation, gonadal development, and maintenance of progenitor cell populations.

Structurally, SOX9 contains a conserved high mobility group DNA-binding domain that enables sequence-specific DNA interaction and induces bending of target promoter regions. This conformational change facilitates recruitment of transcriptional co-activators and chromatin remodeling complexes, allowing activation of downstream genes. SOX9 also contains transactivation domains that interact with regulatory proteins to coordinate tissue-specific transcriptional responses. The SOX9 gene is located on chromosome 17q24.3 and is tightly regulated during embryogenesis and postnatal development.

In skeletal biology, SOX9 is indispensable for chondrogenesis. It activates key extracellular matrix genes such as COL2A1 and ACAN, promoting differentiation of mesenchymal cells into chondrocytes and supporting cartilage maturation. SOX9 expression persists in growth plate cartilage and is required to maintain chondrocyte phenotype. Disruption of SOX9 function leads to severe skeletal abnormalities, most notably campomelic dysplasia, highlighting its central developmental role.

SOX9 is also critical for male sex determination. Acting downstream of SRY in the developing gonad, SOX9 drives Sertoli cell differentiation and testis cord formation. Altered SOX9 expression can result in disorders of sex development. Beyond embryogenesis, SOX9 marks epithelial progenitor populations in tissues including pancreas, bile ducts, lung, and intestinal crypts, where it contributes to regenerative and repair processes.

In oncology research, SOX9 expression has been reported in colorectal, pancreatic, breast, and lung carcinomas. Elevated nuclear SOX9 may be associated with tumor cell plasticity, stem-like features, and epithelial-mesenchymal transition programs. Because SOX9 functions as a transcription factor, immunohistochemical detection typically demonstrates nuclear staining in positive cells.

SOX9 Antibody is suitable for detecting SOX9 protein expression in research applications examining developmental biology, stem cell regulation, skeletal formation, and transcriptional changes associated with disease.

Application Notes

Optimal dilution of the SOX9 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 393-508 was used as the immunogen for the SOX9 antibody.

Storage

Aliquot the SOX9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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