- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Related Products
|
SOX9 Antibody recognizes SRY-box transcription factor 9, encoded by the SOX9 gene and commonly referred to as SRY-box 9. SOX9 is a nuclear transcription factor belonging to the SRY-related HMG-box family and functions as a master regulator of lineage specification, differentiation, and developmental patterning. SOX9 Antibody detects a DNA-binding protein that localizes to the nucleus and controls gene expression programs essential for cartilage formation, gonadal development, and maintenance of progenitor cell populations.
Structurally, SOX9 contains a conserved high mobility group DNA-binding domain that enables sequence-specific DNA interaction and induces bending of target promoter regions. This conformational change facilitates recruitment of transcriptional co-activators and chromatin remodeling complexes, allowing activation of downstream genes. SOX9 also contains transactivation domains that interact with regulatory proteins to coordinate tissue-specific transcriptional responses. The SOX9 gene is located on chromosome 17q24.3 and is tightly regulated during embryogenesis and postnatal development.
In skeletal biology, SOX9 is indispensable for chondrogenesis. It activates key extracellular matrix genes such as COL2A1 and ACAN, promoting differentiation of mesenchymal cells into chondrocytes and supporting cartilage maturation. SOX9 expression persists in growth plate cartilage and is required to maintain chondrocyte phenotype. Disruption of SOX9 function leads to severe skeletal abnormalities, most notably campomelic dysplasia, highlighting its central developmental role.
SOX9 is also critical for male sex determination. Acting downstream of SRY in the developing gonad, SOX9 drives Sertoli cell differentiation and testis cord formation. Altered SOX9 expression can result in disorders of sex development. Beyond embryogenesis, SOX9 marks epithelial progenitor populations in tissues including pancreas, bile ducts, lung, and intestinal crypts, where it contributes to regenerative and repair processes.
In oncology research, SOX9 expression has been reported in colorectal, pancreatic, breast, and lung carcinomas. Elevated nuclear SOX9 may be associated with tumor cell plasticity, stem-like features, and epithelial-mesenchymal transition programs. Because SOX9 functions as a transcription factor, immunohistochemical detection typically demonstrates nuclear staining in positive cells.
SOX9 Antibody is suitable for detecting SOX9 protein expression in research applications examining developmental biology, stem cell regulation, skeletal formation, and transcriptional changes associated with disease.
Optimal dilution of the SOX9 antibody should be determined by the researcher.
A portion of amino acids 393-508 was used as the immunogen for the SOX9 antibody.
Aliquot the SOX9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.