SOX9 Antibody / Cancer Stem Cell Marker Antibody [clone SOX9/2387] (V8917)

SOX9 Antibody Human PC-3 Cancer Cell WB. Western blot analysis of human PC-3 prostate cancer cell lysate using SOX9 Antibody. Recombinant monoclonal antibody clone SOX9/2387 detects a distinct band at approximately 56-65 kDa, consistent with the predicted molecular weight of SOX9. SOX9 is a nuclear transcription factor associated with cancer stem cell regulation and differentiation, and expression in PC-3 cells aligns with its role in tumor progression. Minor variation in apparent molecular weight may reflect post-translational modifications or protein processing.

SOX9 Antibody Pancreas IHC. Immunohistochemistry analysis of FFPE human pancreatic tissue using SOX9 Antibody. The recombinant monoclonal antibody clone SOX9/2387 demonstrates strong nuclear HRP-DAB brown staining in ductal epithelial cells and scattered progenitor-like cell populations, consistent with SOX9’s role as a transcription factor and cancer stem cell-associated marker. Minimal staining is observed in surrounding stromal components. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer, supporting robust nuclear signal detection in pancreatic tissue.

IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SOX9 Antibody Gastric Cancer IHC. Immunohistochemistry analysis of FFPE human gastric carcinoma tissue using SOX9 Antibody. The recombinant monoclonal antibody clone SOX9/2387 demonstrates strong nuclear HRP-DAB brown staining in tumor epithelial cells, with focal enrichment in gland-forming regions, consistent with SOX9 expression in proliferative and progenitor-like tumor compartments. Surrounding stromal cells show minimal staining. The observed pattern supports SOX9’s role as a cancer stem cell-associated transcription factor in gastric carcinoma. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer to optimize nuclear antigen detection.

SDS-PAGE analysis of purified, BSA-free SOX9 antibody (clone SOX9/2387) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX9 antibody (clone SOX9/2387). These results demonstrate the foremost specificity of the SOX9/2387 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.