SOX4 Antibody PCRP-SOX4-1D6 / SOX4 Monoclonal Antibody [clone PCRP-SOX4-1D6] (V9701)

SOX4 Antibody PCRP-SOX4-1D6 by IHC in human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human breast carcinoma tissue using SOX4 Antibody clone PCRP-SOX4-1D6. Brown immunoreactivity is visible in groups of carcinoma cells, with staining involving nuclear and cytoplasmic regions. The distribution is heterogeneous, with some tumor cells showing stronger staining than adjacent cells. Hematoxylin counterstaining highlights nuclei in blue. The SOX4 Monoclonal Antibody was applied following heat-induced epitope retrieval in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 minutes, followed by cooling.

SOX4 Antibody PCRP-SOX4-1D6 by IHC in human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma tissue using SOX4 Antibody clone PCRP-SOX4-1D6. Brown immunoreactivity is distributed among epithelial cells forming glandular structures, with staining concentrated in portions of the glandular epithelium. The surrounding stromal tissue exhibits comparatively limited staining. Hematoxylin counterstaining provides blue nuclear contrast. The SOX4 Monoclonal Antibody was used following heat-induced epitope retrieval in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 minutes, followed by cooling.

SOX4 Antibody PCRP-SOX4-1D6 by IF in HeLa cells. Immunofluorescence staining of paraformaldehyde-fixed human HeLa cells using SOX4 Antibody clone PCRP-SOX4-1D6, detected in green, with phalloidin counterstaining of filamentous actin in red. Green fluorescence is visible predominantly throughout the cytoplasmic regions of the cells, with a relatively diffuse distribution and limited apparent nuclear enrichment. The red phalloidin signal outlines the actin cytoskeleton and cellular boundaries. Although SOX4 is primarily characterized as a nuclear transcription factor, this image does not demonstrate a predominantly nuclear staining pattern. The observed distribution should therefore be interpreted cautiously when assessing the specificity of the SOX4 Monoclonal Antibody.

SOX4 Antibody PCRP-SOX4-1D6 by FACS in HeLa cells. Flow cytometric analysis of paraformaldehyde-fixed human HeLa cells using SOX4 Antibody clone PCRP-SOX4-1D6. The blue histogram represents antibody-stained cells, while the red histogram represents unstained cells. A rightward fluorescence shift is observed in the antibody-stained population, indicating increased fluorescence relative to the unstained control. The SOX4 Monoclonal Antibody was used to evaluate intracellular antigen detection by flow cytometry. This result demonstrates antibody-associated fluorescence under the tested conditions but does not independently establish discrimination between SOX4 and closely related SOXC family proteins.

SOX4 Antibody PCRP-SOX4-1D6 purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free SOX4 Antibody clone PCRP-SOX4-1D6 under reduced (R) and non-reduced (NR) conditions. The reduced sample shows antibody chains migrating near the expected molecular weights of approximately 50 kDa and 25 kDa, corresponding to immunoglobulin heavy and light chains. The non-reduced sample exhibits a prominent higher-molecular-weight band near 150 kDa, consistent with intact immunoglobulin. Molecular weight standards are shown in the left lane. This analysis evaluates the integrity and purity of the SOX4 Monoclonal Antibody preparation rather than detection of endogenous SOX4 protein.

SOX4 Antibody PCRP-SOX4-1D6 by protein microarray. Protein Microarray Validated analysis of SOX4 Antibody clone PCRP-SOX4-1D6 using a HuProt human protein microarray containing more than 19,000 full-length proteins. The graph displays prominent binding signals for SOX4 and SOX12, with substantially lower signals for most other proteins represented in the analysis. The SOX4 Monoclonal Antibody therefore demonstrates recognition of its intended target but also measurable cross-reactivity with SOX12, another member of the SOXC transcription factor family. This result is particularly relevant when interpreting experiments involving tissues or cell populations that express both proteins, since antibody binding cannot be assumed to be exclusive to SOX4.