SOX4 Rabbit Polyclonal Antibody / SRY Box 4 Antibody (F47903)

SOX4 Rabbit Polyclonal Antibody by IF in HeLa cells. Immunofluorescence staining of human HeLa cells using SOX4 Rabbit Polyclonal Antibody, with Alexa Fluor 488-conjugated secondary antibody detection in green. The image shows green fluorescence distributed in punctate and granular structures within the cytoplasm, with comparatively weak nuclear staining. Red fluorescence outlines the cytoskeletal architecture, while nuclei are counterstained blue. The extranuclear distribution differs from the predominantly nuclear localization expected for SOX4 as a transcription factor. Although the product description associates the green signal with mitochondria, the image alone does not establish mitochondrial localization or confirm that the observed cytoplasmic staining represents endogenous SOX4.

SOX4 Rabbit Polyclonal Antibody by IHC in human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded human brain tissue using SOX4 Rabbit Polyclonal Antibody. Brown immunoreactivity is visible in scattered cells throughout the tissue section, with staining involving cellular regions surrounding darker nuclei. Some cells exhibit stronger staining than adjacent tissue elements, producing a heterogeneous distribution. The SRY Box 4 Antibody is used here to examine immunoreactivity in neural tissue, where SOX4 participates in developmental transcriptional regulation. The observed staining pattern should be interpreted alongside appropriate specificity controls because the image does not independently establish the identity of the detected antigen.

SOX4 Rabbit Polyclonal Antibody by FACS in A2058 cells. Flow cytometric analysis of human A2058 cells using SOX4 Rabbit Polyclonal Antibody and a FITC-conjugated goat anti-rabbit secondary antibody. The green histogram represents antibody-stained cells, while the blue histogram represents the negative control. A pronounced rightward fluorescence shift is observed in the antibody-stained population, indicating increased fluorescence relative to the control. The SRY Box 4 Antibody produces a distinct positive signal under the tested conditions, supporting its use in flow cytometric analysis. However, this experiment does not independently establish specificity against other SOXC transcription factors.

SOX4 Rabbit Polyclonal Antibody by WB in A2058 cells. Western blot analysis of human A2058 cell lysate using SOX4 Rabbit Polyclonal Antibody. A band is detected near 47 kDa, consistent with the expected molecular weight of human SOX4. Additional bands are visible at lower molecular weights, including signals near approximately 30 kDa and 20 kDa. The identities of these additional bands have not been established. The SRY Box 4 Antibody demonstrates recognition of a protein migrating near the predicted SOX4 molecular weight, although the presence of additional bands should be considered when evaluating antibody specificity and interpreting western blot results.