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Home >> Antibodies >> SOX2 Antibody

SOX2 Antibody (F54738)

  Catalog No Formulation Size Price (USD)  
Image F54738-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F54738-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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Western blot testing of human NCCIT cell lysate with SOX2 antibody. Predicted molecular weight ~34 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with SOX2 antibody.
Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with SOX2 antibody (green) and anti-Actin (red).
Flow cytometry testing of human HEK293 cells with SOX2 antibody; Blue=isotype control, Green= SOX2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P48431
Localization Nuclear
Applications Immunofluorescence : 1:25
Western Blot : 1:500-1:2000
Flow Cytometry : 1:25 (1x10e6 cells)
Limitations This SOX2 antibody is available for research use only.
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Description

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.

Application Notes

The stated application concentrations are suggested starting points. Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 251-284 from the human protein was used as the immunogen for the SOX2 antibody.

Storage

Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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