SOX10 Polyclonal Antibody (RQ8265)

SOX10 Polyclonal Antibody by IF in Human U-2 OS Cells. Immunofluorescence staining of FFPE human U-2 OS cells using rabbit polyclonal SOX10 antibody. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer at pH 6 for 20 minutes. Red fluorescence is concentrated predominantly within cell nuclei, with a heterogeneous, finely punctate distribution and comparatively little cytoplasmic staining. This pattern is consistent with the nuclear localization of SRY-Box Transcription Factor 10.

SOX10 Polyclonal Antibody by WB in Human, Rat, and Mouse Samples. Western blot analysis using rabbit polyclonal SOX10 antibody. Lane 1: human A375 melanoma cell lysate; lane 2: rat brain lysate; lane 3: mouse brain lysate. Prominent immunoreactive bands are detected at approximately 75-80 kDa in all three samples, above the expected molecular weight of 50-58 kDa for SRY-Box Transcription Factor 10. The human A375 sample also exhibits a weaker band at a slightly lower molecular weight. SOX10 has been reported to undergo phosphorylation and exhibit multiple electrophoretic forms, which may contribute to differences in apparent molecular weight.

SOX10 Polyclonal Antibody by FACS in Human PC-3 Cells. Flow cytometry analysis of fixed and permeabilized human PC-3 cells using rabbit polyclonal SOX10 antibody at 1 ug per million cells. Cells were blocked with goat serum before antibody staining. The histogram compares SOX10 antibody staining (blue), isotype control (green), and unstained cells (red). The antibody-stained population exhibits a pronounced rightward fluorescence shift relative to both controls, demonstrating intracellular detection of SRY-Box Transcription Factor 10.