SNRNP200 Antibody | U5 snRNP Helicase

	Catalog No	Formulation	Size	Price (USD)
	RQ7774	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

SNRNP200 Antibody by IF in Human Intestinal Cancer Tissue. Immunofluorescence staining of formalin-fixed, paraffin-embedded human intestinal carcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by steaming tissue sections in EDTA buffer at pH 8.0 for 20 minutes. U5 snRNP 200 kDa Helicase immunoreactivity is visualized in red, with DAPI nuclear counterstaining in blue. The merged image demonstrates prominent nuclear-associated fluorescence throughout the malignant epithelial tissue, consistent with the nuclear localization of this spliceosomal RNA helicase.

SNRNP200 Antibody by IF in Human Caco-2 Cells. Immunofluorescence staining of formalin-fixed, paraffin-embedded human Caco-2 cells using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by steaming in citrate buffer at pH 6.0 for 20 minutes. U5 snRNP 200 kDa Helicase is visualized in red, while beta-tubulin is labeled in green. Strong red fluorescence is concentrated within the nuclei, with green beta-tubulin staining outlining the surrounding cytoplasmic microtubule network. The distinct nuclear and cytoplasmic staining patterns demonstrate the subcellular distribution of SNRNP200 relative to beta-tubulin.

SNRNP200 Antibody by IHC in Human Endometrioid Adenocarcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human endometrioid adenocarcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling before testing. Prominent brown DAB staining is visible in the nuclei of malignant glandular epithelial cells, with comparatively weaker staining in surrounding stromal elements. This nuclear distribution is consistent with the role of U5 snRNP 200 kDa Helicase in pre-mRNA splicing.

SNRNP200 Antibody by IHC in Human Diffuse Large B-Cell Lymphoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human diffuse large B-cell lymphoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is distributed among the nuclei of malignant lymphoid cells, with variation in staining intensity across the tissue. The results demonstrate nuclear-associated expression of U5 snRNP 200 kDa Helicase in human lymphoma tissue.

SNRNP200 Antibody by IHC in Human Liver Cancer Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is observed in tumor cell nuclei, with heterogeneous staining intensity across the malignant tissue. The nuclear labeling supports detection of U5 snRNP 200 kDa Helicase, a component of the spliceosomal machinery involved in RNA processing.

SNRNP200 Antibody by IHC in Human Testicular Seminoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human testicular seminoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is visible in numerous tumor cell nuclei, with variation in intensity throughout the section. This pattern demonstrates expression of U5 snRNP 200 kDa Helicase in seminoma tissue.

SNRNP200 Antibody by IHC in Human Spleen Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human spleen tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown nuclear staining is visible in a subset of splenic cells, with variable intensity across the lymphoid tissue. The results demonstrate detection of U5 snRNP 200 kDa Helicase in human spleen.

SNRNP200 Antibody by IHC in Human Prostate Adenocarcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Prominent brown DAB staining is observed in the nuclei of glandular epithelial cells, with comparatively weaker staining in portions of the surrounding stroma. This distribution demonstrates nuclear expression of U5 snRNP 200 kDa Helicase in prostate carcinoma tissue.

SNRNP200 Antibody by IHC in Human Lung Adenocarcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is prominent in the nuclei of malignant epithelial cells, while surrounding inflammatory and stromal cells show variable labeling. The results demonstrate U5 snRNP 200 kDa Helicase expression in human lung adenocarcinoma.

SNRNP200 Antibody by IHC in Human Glioblastoma Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human glioblastoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is distributed among numerous tumor cell nuclei, with heterogeneous staining intensity throughout the section. This nuclear pattern is consistent with the localization of U5 snRNP 200 kDa Helicase and demonstrates SNRNP200 expression in glioblastoma tissue.

SNRNP200 Antibody by IHC in Mouse Brain Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded mouse brain tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Scattered brown DAB-positive nuclei are visible throughout the brain tissue, with variation in staining intensity among individual cells. The results demonstrate detection of U5 snRNP 200 kDa Helicase in mouse neural tissue.

SNRNP200 Antibody by IHC in Rat Brain Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded rat brain tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown nuclear staining is visible in scattered cells throughout the brain section, with relatively low background staining in the surrounding tissue. The results demonstrate expression of U5 snRNP 200 kDa Helicase in rat neural tissue.

SNRNP200 Antibody by WB in Human 293T Cell Lysate. Western blot analysis of human 293T whole-cell lysate using rabbit polyclonal SNRNP200 antibody. A distinct band is detected in the high-molecular-weight region, approximately 200-250 kDa, consistent with the predicted molecular weight of approximately 245 kDa for SNRNP200. The protein is also known as U5 snRNP 200 kDa Helicase, although its calculated molecular weight is higher than its historical designation. The result demonstrates detection of this large spliceosomal RNA helicase in human cells.

SNRNP200 Antibody by FACS in Human A431 Cells. Flow cytometry analysis of human A431 cells using rabbit polyclonal SNRNP200 antibody at 1 ug per million cells following blocking with goat serum. The histogram compares cells alone (red), isotype control (green), and SNRNP200 antibody staining (blue). The antibody-stained population demonstrates a rightward fluorescence shift relative to the unstained control, with modest separation from the isotype control. This result supports detection of U5 snRNP 200 kDa Helicase in human A431 cells.

SNRNP200 Antibody by FACS in Mouse ANA-1 Cells. Flow cytometry analysis of mouse ANA-1 cells using rabbit polyclonal SNRNP200 antibody at 1 ug per million cells following blocking with goat serum. The histogram compares cells alone (red), isotype control (green), and SNRNP200 antibody staining (blue). The antibody-stained population shows a rightward fluorescence shift relative to the unstained control, with limited separation from the isotype control. The results support detection of U5 snRNP 200 kDa Helicase in mouse ANA-1 cells.

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SNRNP200 Antibody by IF in Human Intestinal Cancer Tissue. Immunofluorescence staining of formalin-fixed, paraffin-embedded human intestinal carcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by steaming tissue sections in EDTA buffer at pH 8.0 for 20 minutes. U5 snRNP 200 kDa Helicase immunoreactivity is visualized in red, with DAPI nuclear counterstaining in blue. The merged image demonstrates prominent nuclear-associated fluorescence throughout the malignant epithelial tissue, consistent with the nuclear localization of this spliceosomal RNA helicase.

SNRNP200 Antibody by IF in Human Caco-2 Cells. Immunofluorescence staining of formalin-fixed, paraffin-embedded human Caco-2 cells using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by steaming in citrate buffer at pH 6.0 for 20 minutes. U5 snRNP 200 kDa Helicase is visualized in red, while beta-tubulin is labeled in green. Strong red fluorescence is concentrated within the nuclei, with green beta-tubulin staining outlining the surrounding cytoplasmic microtubule network. The distinct nuclear and cytoplasmic staining patterns demonstrate the subcellular distribution of SNRNP200 relative to beta-tubulin.

SNRNP200 Antibody by IHC in Human Endometrioid Adenocarcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human endometrioid adenocarcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling before testing. Prominent brown DAB staining is visible in the nuclei of malignant glandular epithelial cells, with comparatively weaker staining in surrounding stromal elements. This nuclear distribution is consistent with the role of U5 snRNP 200 kDa Helicase in pre-mRNA splicing.

SNRNP200 Antibody by IHC in Human Diffuse Large B-Cell Lymphoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human diffuse large B-cell lymphoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is distributed among the nuclei of malignant lymphoid cells, with variation in staining intensity across the tissue. The results demonstrate nuclear-associated expression of U5 snRNP 200 kDa Helicase in human lymphoma tissue.

SNRNP200 Antibody by IHC in Human Liver Cancer Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is observed in tumor cell nuclei, with heterogeneous staining intensity across the malignant tissue. The nuclear labeling supports detection of U5 snRNP 200 kDa Helicase, a component of the spliceosomal machinery involved in RNA processing.

SNRNP200 Antibody by IHC in Human Testicular Seminoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human testicular seminoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is visible in numerous tumor cell nuclei, with variation in intensity throughout the section. This pattern demonstrates expression of U5 snRNP 200 kDa Helicase in seminoma tissue.

SNRNP200 Antibody by IHC in Human Spleen Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human spleen tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown nuclear staining is visible in a subset of splenic cells, with variable intensity across the lymphoid tissue. The results demonstrate detection of U5 snRNP 200 kDa Helicase in human spleen.

SNRNP200 Antibody by IHC in Human Prostate Adenocarcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Prominent brown DAB staining is observed in the nuclei of glandular epithelial cells, with comparatively weaker staining in portions of the surrounding stroma. This distribution demonstrates nuclear expression of U5 snRNP 200 kDa Helicase in prostate carcinoma tissue.

SNRNP200 Antibody by IHC in Human Lung Adenocarcinoma. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is prominent in the nuclei of malignant epithelial cells, while surrounding inflammatory and stromal cells show variable labeling. The results demonstrate U5 snRNP 200 kDa Helicase expression in human lung adenocarcinoma.

SNRNP200 Antibody by IHC in Human Glioblastoma Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human glioblastoma tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown DAB staining is distributed among numerous tumor cell nuclei, with heterogeneous staining intensity throughout the section. This nuclear pattern is consistent with the localization of U5 snRNP 200 kDa Helicase and demonstrates SNRNP200 expression in glioblastoma tissue.

SNRNP200 Antibody by IHC in Mouse Brain Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded mouse brain tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Scattered brown DAB-positive nuclei are visible throughout the brain tissue, with variation in staining intensity among individual cells. The results demonstrate detection of U5 snRNP 200 kDa Helicase in mouse neural tissue.

SNRNP200 Antibody by IHC in Rat Brain Tissue. Immunohistochemistry staining of formalin-fixed, paraffin-embedded rat brain tissue using rabbit polyclonal SNRNP200 antibody. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer at pH 8.0 for 20 minutes, followed by cooling. Brown nuclear staining is visible in scattered cells throughout the brain section, with relatively low background staining in the surrounding tissue. The results demonstrate expression of U5 snRNP 200 kDa Helicase in rat neural tissue.

SNRNP200 Antibody by WB in Human 293T Cell Lysate. Western blot analysis of human 293T whole-cell lysate using rabbit polyclonal SNRNP200 antibody. A distinct band is detected in the high-molecular-weight region, approximately 200-250 kDa, consistent with the predicted molecular weight of approximately 245 kDa for SNRNP200. The protein is also known as U5 snRNP 200 kDa Helicase, although its calculated molecular weight is higher than its historical designation. The result demonstrates detection of this large spliceosomal RNA helicase in human cells.

SNRNP200 Antibody by FACS in Human A431 Cells. Flow cytometry analysis of human A431 cells using rabbit polyclonal SNRNP200 antibody at 1 ug per million cells following blocking with goat serum. The histogram compares cells alone (red), isotype control (green), and SNRNP200 antibody staining (blue). The antibody-stained population demonstrates a rightward fluorescence shift relative to the unstained control, with modest separation from the isotype control. This result supports detection of U5 snRNP 200 kDa Helicase in human A431 cells.

SNRNP200 Antibody by FACS in Mouse ANA-1 Cells. Flow cytometry analysis of mouse ANA-1 cells using rabbit polyclonal SNRNP200 antibody at 1 ug per million cells following blocking with goat serum. The histogram compares cells alone (red), isotype control (green), and SNRNP200 antibody staining (blue). The antibody-stained population shows a rightward fluorescence shift relative to the unstained control, with limited separation from the isotype control. The results support detection of U5 snRNP 200 kDa Helicase in mouse ANA-1 cells.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	O75643
Localization	Nuclear
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This SNRNP200 Antibody / U5 snRNP 200 kDa Helicase Antibody is available for research use only.
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Description

SNRNP200 Antibody recognizes small nuclear ribonucleoprotein U5 subunit 200 (SNRNP200), a large ATP-dependent RNA helicase essential for pre-mRNA splicing. Also known as BRR2, ASCC3L1, and U5 snRNP 200 kDa helicase, this protein is a core component of the U5 small nuclear ribonucleoprotein complex. SNRNP200 belongs to the DExH-box family of RNA helicases and plays a central role in the structural rearrangements required for spliceosome activation and catalytic function.

The spliceosome is a dynamic ribonucleoprotein assembly responsible for removing introns from precursor messenger RNA and joining exons to produce mature transcripts. Its major components include the U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins, together with numerous associated regulatory proteins. U5 snRNP participates in positioning RNA substrates during splicing, while the U5 snRNP 200 kDa helicase provides ATP-dependent RNA remodeling activity needed for progression through the splicing cycle.

During spliceosome assembly, U4 and U6 small nuclear RNAs form a duplex that must be unwound before the catalytic RNA network can become active. BRR2 promotes unwinding of the U4/U6 duplex, allowing U6 RNA to participate in the rearrangements necessary for formation of the active spliceosome. This activity is tightly regulated because premature or inappropriate RNA unwinding can interfere with accurate intron removal and transcript maturation.

SNRNP200 contains two helicase-like modules arranged in tandem, although its principal RNA-unwinding activity resides in the N-terminal module. Interactions with other spliceosomal proteins, including PRPF8 and EFTUD2, help coordinate helicase activity with spliceosome assembly and activation. These regulatory relationships contribute to the fidelity and timing of pre-mRNA processing.

Pathogenic variants in SNRNP200 are associated with autosomal dominant retinitis pigmentosa, a hereditary retinal degeneration characterized by progressive photoreceptor dysfunction. Certain disease-associated variants impair RNA helicase activity or disrupt spliceosomal regulation, highlighting the importance of accurate RNA processing in retinal cell maintenance. SNRNP200 is therefore relevant to investigations of inherited retinal disease, spliceosome function, and RNA metabolism.

An SNRNP200 Antibody from NSJ Bioreagents supports research investigating U5 snRNP 200 kDa Helicase expression, BRR2-mediated RNA remodeling, spliceosome activation, and molecular mechanisms underlying pre-mRNA splicing and retinal degeneration.

For antibodies targeting additional proteins involved in RNA processing and other essential cellular functions, explore our Cell Biology Antibodies collection.

Application Notes

Optimal dilution of the SNRNP200 Antibody / U5 snRNP 200 kDa Helicase Antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids K557-A2129) was used as the immunogen for the SNRNP200 antibody.

Storage

After reconstitution, the SNRNP200 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

U5 snRNP 200 kDa Helicase antibody, BRR2 antibody, U5 snRNP-Specific 200 kDa Protein antibody, U5 Small Nuclear Ribonucleoprotein 200 kDa Helicase antibody, ASCC3L1 antibody, SNRNP200 RNA Helicase antibody, U5-200K antibody

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SNRNP200 Antibody / U5 snRNP 200 kDa Helicase Antibody (RQ7774)

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