SMC4 Antibody | CAP-C Antibody | Condensin Protein

	Catalog No	Formulation	Size	Price (USD)
	RQ8909	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

SMC4 Antibody detection in HeLa cells by IF. Immunofluorescence staining of FFPE human HeLa cells using the SMC4 Antibody (green) with Alpha Tubulin antibody (red). Strong green SMC4 signal is concentrated predominantly within the nuclei, with additional weaker cytoplasmic fluorescence visible in some cells. The red microtubule network provides a clear contrast to the nuclear-enriched SMC4 distribution. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 minutes. The observed intracellular staining demonstrates detection of chromosome-associated polypeptide C with this CAP-C Antibody.

SMC4 Antibody detection in human cell lines by WB. Western blot testing of human U-251 (lane 1), K562 (lane 2) and PC-3 (lane 3) cell lysates with the SMC4 Antibody. A strong predominant band is detected at approximately 150 kDa in all three samples, closely corresponding to the predicted molecular weight of approximately 147 kDa for SMC4. A weaker lower-molecular-weight band is visible in the U-251 lane but is not prominent in K562 or PC-3. The consistent major band across all three lysates supports detection of structural maintenance of chromosomes protein 4 with this CAP-C Antibody.

SMC4 Antibody detection in PC-3 cells by FACS. Flow cytometry analysis of fixed and permeabilized human PC-3 cells using the SMC4 Antibody at 1 ug per million cells. The blue antibody-treated population shows a clear rightward fluorescence shift relative to the green isotype control and red cells-alone population, demonstrating specific intracellular detection of SMC4. Cells were blocked with goat serum before testing. The distinct separation of the antibody-treated population supports recognition of chromosome-associated polypeptide C by this CAP-C Antibody.

SMC4 Antibody staining of human kidney by IHC. Immunohistochemistry of FFPE human kidney sections using the SMC4 Antibody at 2 ug/ml. Brown staining is visible in subsets of renal tubular epithelial cells, with signal present in both nuclear and cytoplasmic compartments and variation in intensity among individual tubules. Glomerular and interstitial regions show comparatively limited staining, while nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH 8 EDTA before antibody incubation. The observed tissue staining demonstrates detection of structural maintenance of chromosomes protein 4 with this CAP-C Antibody.

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SMC4 Antibody detection in HeLa cells by IF. Immunofluorescence staining of FFPE human HeLa cells using the SMC4 Antibody (green) with Alpha Tubulin antibody (red). Strong green SMC4 signal is concentrated predominantly within the nuclei, with additional weaker cytoplasmic fluorescence visible in some cells. The red microtubule network provides a clear contrast to the nuclear-enriched SMC4 distribution. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 minutes. The observed intracellular staining demonstrates detection of chromosome-associated polypeptide C with this CAP-C Antibody.

SMC4 Antibody detection in human cell lines by WB. Western blot testing of human U-251 (lane 1), K562 (lane 2) and PC-3 (lane 3) cell lysates with the SMC4 Antibody. A strong predominant band is detected at approximately 150 kDa in all three samples, closely corresponding to the predicted molecular weight of approximately 147 kDa for SMC4. A weaker lower-molecular-weight band is visible in the U-251 lane but is not prominent in K562 or PC-3. The consistent major band across all three lysates supports detection of structural maintenance of chromosomes protein 4 with this CAP-C Antibody.

SMC4 Antibody detection in PC-3 cells by FACS. Flow cytometry analysis of fixed and permeabilized human PC-3 cells using the SMC4 Antibody at 1 ug per million cells. The blue antibody-treated population shows a clear rightward fluorescence shift relative to the green isotype control and red cells-alone population, demonstrating specific intracellular detection of SMC4. Cells were blocked with goat serum before testing. The distinct separation of the antibody-treated population supports recognition of chromosome-associated polypeptide C by this CAP-C Antibody.

SMC4 Antibody staining of human kidney by IHC. Immunohistochemistry of FFPE human kidney sections using the SMC4 Antibody at 2 ug/ml. Brown staining is visible in subsets of renal tubular epithelial cells, with signal present in both nuclear and cytoplasmic compartments and variation in intensity among individual tubules. Glomerular and interstitial regions show comparatively limited staining, while nuclei are counterstained blue. Heat-mediated antigen retrieval was performed in pH 8 EDTA before antibody incubation. The observed tissue staining demonstrates detection of structural maintenance of chromosomes protein 4 with this CAP-C Antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q9NTJ3
Localization	Nuclear, cytoplasmic
Applications	Western Blot : 0.5-1ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations	This SMC4 Antibody / CAP-C Antibody is available for research use only.
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Description

SMC4 Antibody / CAP-C Antibody recognizes structural maintenance of chromosomes protein 4, a large ATPase encoded by the SMC4 gene. Also known as CAP-C, hCAP-C and SMC4L1, SMC4 belongs to the structural maintenance of chromosomes family of proteins that organize and regulate higher-order chromosome architecture. SMC4 associates with SMC2 to form the central heterodimeric scaffold of the condensin I and condensin II complexes. These complexes are essential for the extensive structural reorganization of chromosomes that accompanies cell division.

SMC4 contains conserved ATPase domains separated by an extended coiled-coil region and a central hinge domain. Folding of the protein brings its N- and C-terminal ATPase regions together, while the hinge facilitates association with SMC2. The resulting SMC2-SMC4 complex interacts with additional condensin subunits to regulate chromosome topology. ATP-dependent conformational changes within the complex contribute to chromosome compaction and organization. A SMC4 Antibody can therefore support research into condensin function and the molecular mechanisms responsible for chromosome architecture.

During mitosis, condensin complexes participate in chromosome condensation, resolution of sister chromatids and accurate chromosome segregation. SMC4 function is also associated with maintenance of genomic integrity and cellular responses to DNA damage. Disruption of condensin activity can produce abnormal chromosome morphology, segregation defects and genomic instability. CAP-C Antibody research is therefore relevant to studies of cell-cycle progression, chromosome dynamics and mechanisms that preserve genome organization during proliferative growth.

Altered SMC4 expression has been investigated in multiple cancers, where increased expression has been associated with enhanced proliferation and aggressive tumor characteristics. These observations have generated interest in SMC4 as a component of the chromosome-maintenance machinery linking condensin activity with cancer cell growth and genomic instability. The fundamental role of SMC4 in chromosome organization also makes the protein relevant to broader studies of mitosis, meiosis and nuclear architecture. NSJ Bioreagents provides this polyclonal reagent for researchers investigating condensin biology and chromosome maintenance. An SMC4 Antibody provides a useful tool for studying CAP-C and its roles in chromosome condensation, segregation and genomic stability.

This SMC4 Antibody is part of our Epigenetics Antibodies collection.

Application Notes

Optimal dilution of the SMC4 Antibody / CAP-C Antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K911-C1288) was used as the immunogen for the SMC4 Antibody.

Storage

After reconstitution, the SMC4 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

CAP-C antibody, hCAP-C antibody, SMC4L1 antibody, Structural maintenance of chromosomes protein 4 antibody, Chromosome-associated polypeptide C antibody

SMC4 Antibody / CAP-C Antibody (RQ8909)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents