SMAD3 Antibody / TGF-beta Signaling Protein Antibody [clone PCRP-SMAD3-1A2] (V9231)

SMAD3 Antibody HeLa Cell IF. Immunofluorescence staining was performed on paraformaldehyde-fixed human HeLa cells using SMAD3 Antibody clone PCRP-SMAD3-1A2 (green). F-actin was counterstained with phalloidin (red). Distinct cytoplasmic and nuclear staining is observed, consistent with the intracellular distribution of SMAD3, while phalloidin clearly delineates the actin cytoskeleton and cell morphology. These results demonstrate that this TGF-beta Signaling Protein Antibody, a Protein Microarray Validated mouse monoclonal, is suitable for immunofluorescent detection of SMAD3 in cultured human cells.

SMAD3 Antibody HeLa Cell FACS. Flow cytometric analysis was performed on paraformaldehyde-fixed human HeLa cells using SMAD3 Antibody clone PCRP-SMAD3-1A2. The red histogram represents unstained cells, while the blue histogram represents cells stained with the SMAD3 antibody. A clear rightward shift of the stained cell population relative to the unstained control demonstrates specific antibody binding and confirms SMAD3 expression in HeLa cells. These results demonstrate that this TGF-beta Signaling Protein Antibody, a Protein Microarray Validated mouse monoclonal, is suitable for flow cytometric detection of SMAD3.

SMAD3 Antibody Protein Microarray Validation. Specificity of SMAD3 Antibody clone PCRP-SMAD3-1A2 was evaluated using the HuProt Protein Microarray containing more than 19,000 full-length human proteins. SMAD3 ranked as the top target with a Z-score of 102.97 and an S-score of 96.1, demonstrating exceptional specificity relative to all other proteins on the array. These findings confirm that this TGF-beta Signaling Protein Antibody is Protein Microarray Validated, providing researchers with high confidence in target specificity. The Z-score reflects the strength of antibody binding to a given protein relative to the array background, while the S-score measures target specificity by comparing the Z-score of the intended target with that of the next highest-ranking protein.

SDS-PAGE analysis of purified, BSA-free SMAD3 antibody (PCRP-SMAD3-1A2) as confirmation of integrity and purity.