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Home >> Antibodies >> SMAD3 Antibody

SMAD3 Antibody (F43048)

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  Catalog No Formulation Size Price (USD)  
Image F43048-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F43048-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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SMAD3 antibody immunohsitochemistry analysis in formalin fixed and paraffin embedded human heart tissue.
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SMAD3 antibody immunohsitochemistry analysis in formalin fixed and paraffin embedded human heart tissue.
Confocal immunofluorescent analysis of SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
SMAD3 antibody western blot analysis in CEM lysate. Observed molecular weight: 48~55 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Pig, Chicken
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P84022
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
IHC (Paraffin) : 1:10-1:50
Limitations This SMAD3 antibody is available for research use only.
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Description

SMAD3 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

Application Notes

Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 127-156 from the human protein was used as the immunogen for this SMAD3 antibody.

Storage

Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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