SMAD2 Antibody / Mothers Against Decapentaplegic Homolog 2 Antibody (RQ5451)

SMAD2 Antibody WB in HeLa Wild-Type and SMAD2 Knockout Cells. Western blot analysis of human HeLa wild-type (lane 1) and SMAD2 knockout (lane 2) whole-cell lysates using the Mothers Against Decapentaplegic Homolog 2 Antibody. A prominent band at approximately 55-58 kDa is detected in wild-type cells and is substantially reduced in the knockout sample, supporting SMAD2-specific recognition. The predicted molecular weight of SMAD2 is approximately 52 kDa. Samples were loaded at 30 ug per lane and separated by 10% SDS-PAGE under reducing conditions. The primary antibody was used at 0.5 ug/ml, with GAPDH serving as the loading control.

SMAD2 Antibody WB in Human Placenta, Rat Liver, and Mouse Tissues. Western blot analysis of human placenta (lane 1), rat liver (lane 2), mouse testis (lane 3), mouse heart (lane 4), and mouse lung (lanes 5 and 6) tissue lysates using the Mothers Against Decapentaplegic Homolog 2 Antibody. A band is detected at approximately 55-60 kDa in all six lanes, compared with the predicted molecular weight of approximately 52 kDa for SMAD2. Samples were loaded at 50 ug per lane and separated by 5-20% SDS-PAGE under reducing conditions. The primary antibody was used at 0.5 ug/ml.

SMAD2 Antibody IF in Human HeLa Cells. Immunofluorescence staining of human HeLa cells using the Mothers Against Decapentaplegic Homolog 2 Antibody at 5 ug/ml, detected with DyLight 594-conjugated goat anti-rabbit IgG (red), with DAPI nuclear counterstaining (blue). SMAD2 immunoreactivity is observed predominantly in the cytoplasm, with perinuclear enrichment and some apparent nuclear staining. Enzymatic antigen retrieval was performed for 15 minutes, followed by blocking with 10% goat serum. The primary antibody was incubated overnight at 4°C.

SMAD2 Antibody IHC in Human Placenta. Immunohistochemical staining of FFPE human placenta tissue using the Mothers Against Decapentaplegic Homolog 2 Antibody at 1 ug/ml. Brown DAB staining is observed predominantly in trophoblastic cells surrounding the chorionic villi, with comparatively limited staining in the villous stromal regions. Heat-induced epitope retrieval was performed in citrate buffer (pH 6.0) for 20 minutes, followed by blocking with 10% goat serum. The primary antibody was incubated overnight at 4°C, and detection was performed using a biotinylated secondary antibody and streptavidin-biotin complex.

SMAD2 Antibody IHC in Human Colon Cancer. Immunohistochemical staining of FFPE human colon cancer tissue using the Mothers Against Decapentaplegic Homolog 2 Antibody at 1 ug/ml. Brown DAB staining is observed predominantly in the cytoplasm of malignant glandular epithelial cells, with variable nuclear staining and comparatively limited staining in the surrounding stromal tissue. Heat-induced epitope retrieval was performed in citrate buffer (pH 6.0) for 20 minutes, followed by blocking with 10% goat serum. The primary antibody was incubated overnight at 4°C, and detection was performed using a biotinylated secondary antibody and streptavidin-biotin complex.

SMAD2 Antibody IHC in Mouse Brain. Immunohistochemical staining of FFPE mouse brain tissue using the Mothers Against Decapentaplegic Homolog 2 Antibody at 1 ug/ml. Brown DAB staining is observed in numerous cells throughout the brain parenchyma, with prominent nuclear and perinuclear immunoreactivity in cells exhibiting neuronal morphology. Heat-induced epitope retrieval was performed in citrate buffer (pH 6.0) for 20 minutes, followed by blocking with 10% goat serum. The primary antibody was incubated overnight at 4°C, and detection was performed using a biotinylated secondary antibody and streptavidin-biotin complex.

SMAD2 Antibody IHC in Rat Brain. Immunohistochemical staining of FFPE rat brain tissue using the Mothers Against Decapentaplegic Homolog 2 Antibody at 1 ug/ml. Brown DAB staining is observed in the cerebellar cortex, with prominent immunoreactivity in Purkinje cells along the boundary between the molecular and granular layers. Diffuse staining is also visible in the molecular layer, while the densely packed cells of the granular layer show comparatively limited staining. Heat-induced epitope retrieval was performed in citrate buffer (pH 6.0) for 20 minutes, followed by blocking with 10% goat serum. The primary antibody was incubated overnight at 4°C, and detection was performed using a biotinylated secondary antibody and streptavidin-biotin complex.

SMAD2 Antibody FACS in Human K562 Cells. Flow cytometry analysis of fixed and permeabilized human K562 cells using the Mothers Against Decapentaplegic Homolog 2 Antibody at 1 ug per million cells, detected with DyLight 488-conjugated goat anti-rabbit IgG. The antibody-stained population (blue) exhibits a pronounced rightward fluorescence shift relative to the rabbit IgG isotype control (green) and unlabeled cells (red), indicating intracellular SMAD2-associated immunoreactivity. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum before staining.