• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> SMAD2 Antibody

SMAD2 Antibody (F50345)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F50345-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50345-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.
›
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.
SMAD2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of SMAD2 antibody and NIH3T3 lysate. Predicted molecular weight: 52~60 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Rat, Bovine, Pig, Chicken, Zebrafish, Drosophila
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q15796
Applications Immunofluorescence : 1:100
Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This SMAD2 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC-P, FACS
    Reactivity : Human
  • Applications : WB, IHC-P, ICC
    Reactivity : Human
  • Applications : IF, WB, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Bovine, Zebrafish
  • Applications : WB, ELISA
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Bovine, Pig, Chicken
  • Applications : WB, ELISA
    Reactivity : Mouse
  • Applications : IF, DB, ELISA
    Reactivity : Human
    Pred. Reactivity : Bovine, Chicken, Drosophila, Mouse, Pig, Rat, Zebrafish
  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Mouse
    Pred. Reactivity : Cow

Description

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

Application Notes

Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 97-125 from the human protein was used as the immunogen for this SMAD2 antibody.

Storage

Aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all SMAD2 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.