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Home >> Antibodies >> SMAD1 Antibody

SMAD1 Antibody (F52570)

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  Catalog No Formulation Size Price (USD)  
Image F52570-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F52570-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC analysis of FFPE human heart section using SMAD1 antibody; Ab was diluted at 1:25.
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IHC analysis of FFPE human heart section using SMAD1 antibody; Ab was diluted at 1:25.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.
Western blot analysis of lysate from (1) HT-1080, (2) HUVEC, (3) mouse C2C12 cell line and (4) rat liver tissue using SMAD1 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Bovine
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q15797
Applications Immunofluorescence : 1:25
IHC (Paraffin) : 1:25
Western blot : 1:1000
Limitations This SMAD1 antibody is available for research use only.
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Description

SMADs are intracellular proteins that transduce extracellular signals from transforming growth factor beta ligands to the nucleus where they activate downstream gene transcription. The SMADs, which form a trimer of two receptor-regulated SMADs and one co-SMAD, act as transcription factors that regulate the expression of certain genes. There are three classes of SMADs, SMAD1 belongs to the receptor-regulated class (R-SMAD) which includes SMAD2, SMAD3, SMAD5 and SMAD8/9. [Wiki]

Application Notes

Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This SMAD1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.

Storage

Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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