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Home >> Antibodies >> Smac Antibody / Diablo

Smac Antibody / Diablo (R32332)

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  Catalog No Formulation Size Price (USD)  
Image R32332 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with Smac antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with Smac antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human testis with Smac antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse testis with Smac antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat testis with Smac antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) rat testis, 2) human 22RV1 and 3) human SKOV lysate with Smac antibody. Expected/observed molecular weight 21~27 kDa.
Flow cytometry testing of human K562 cells with Smac antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Smac antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q9NR28
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
Limitations This Smac antibody is available for research use only.
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Description

Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the Smac antibody should be determined by the researcher.

Immunogen

Amino acids 56-239 of human Smac/Diablo were used as the immunogen for the Smac antibody.

Storage

After reconstitution, the Smac antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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