SLC7A11 Antibody / XCT / Solute carrier family 7 member 11 (FY12494)

Immunohistochemical staining of xCT/SLC7A11 using anti-SLC7A11 antibody. xCT/SLC7A11 was detected in a paraffin-embedded section of human ovraian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SLC7A11 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Western blot analysis of xCT/SLC7A11 using anti-SLC7A11 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HepG2 whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A11 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. SLC7A11 (~55 kDa predicted) was detected as a single band at ~45-50 kDa, consistent with published reports describing faster migration of this multi-pass membrane transporter due to its hydrophobic transmembrane regions.