SLC4A1 Antibody / Erythrocyte Membrane Protein Antibody [clone 5G2G7] (RQ7114)

SLC4A1 Antibody Human Spleen IHC. Immunohistochemical staining of FFPE human spleen tissue using SLC4A1 Antibody / Erythrocyte Membrane Protein Antibody demonstrates widespread membranous and cytoplasmic HRP-DAB brown staining within erythrocyte-rich cellular populations distributed throughout splenic tissue. SLC4A1 (AE1/Band 3) is a chloride/bicarbonate exchanger that plays a critical role in erythrocyte membrane function, carbon dioxide transport, and maintenance of acid-base homeostasis. As one of the most abundant membrane proteins in red blood cells, SLC4A1 contributes to membrane organization, structural stability, and physiologic ion transport. The observed staining pattern is consistent with the established role of SLC4A1 in erythroid cell biology and blood-associated tissues. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

SLC4A1 Antibody K562 WB. Western blot analysis of human K562 cell lysate using SLC4A1 Antibody / Erythrocyte Membrane Protein Antibody demonstrates a prominent band at approximately 100-110 kDa, consistent with expression of SLC4A1 (AE1/Band 3). SLC4A1 is a chloride/bicarbonate exchanger that plays an essential role in erythrocyte membrane physiology, carbon dioxide transport, and maintenance of cellular ionic balance. The observed molecular weight is consistent with the expected size of SLC4A1 and may be influenced by glycosylation, which is known to affect electrophoretic migration of the mature protein. Detection in K562 cells is consistent with the hematopoietic lineage-associated biology of SLC4A1 and its role in erythroid differentiation and membrane transport pathways.

SLC4A1 Antibody HepG2 FACS. Flow cytometric analysis of human HepG2 cells using SLC4A1 Antibody / Erythrocyte Membrane Protein Antibody demonstrates a pronounced rightward shift of the SLC4A1-positive population (blue) relative to both the isotype control (green) and unstained cells (red), consistent with expression of SLC4A1. Also known as AE1 or Band 3, SLC4A1 functions as a chloride/bicarbonate exchanger involved in ion transport, acid-base regulation, and maintenance of cellular homeostasis. The distinct fluorescence shift supports the utility of this antibody for flow cytometric detection of SLC4A1 expression. Red=cells alone; Green=isotype control; Blue=SLC4A1 antibody.