SLC2A1 Antibody for IF / GLUT1 Glucose Transporter (R31941)

SLC2A1 Antibody for IF staining in human placenta tissue. FFPE human placental section shows green fluorescent staining corresponding to GLUT1 (SLC2A1) localization outlining cell membranes and cytoplasmic vesicular structures, consistent with plasma membrane distribution of the glucose transporter. The fluorescent signal highlights metabolically active placental cells where GLUT1 mediates glucose transport. Nuclei are counterstained with DAPI (blue). Heat-induced antigen retrieval was performed by boiling paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to antibody incubation. Signal was detected using an immunofluorescence method and visualized by fluorescence microscopy.

ICC testing of human A549 cells with SLC2A1 antibody.

IHC testing of FFPE human placenta with SLC2A1 Antibody for IF. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of frozen human placenta with SLC2A1 antibody.

IHC testing of FFPE mouse brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human placenta with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human breast cancer with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE mouse brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat brain with SLC2A1 antibody. HIER: Boil the paraffin sections in pH 8 EDTA for 20 minutes and allow to cool prior to staining.

Western blot testing of 1) human placenta, 2) human A549, 3) mouse brain, 4) rat PC-12 and 5) mouse NIH 3T3 lysate wtih SLC2A1 antibody. Predicted molecular weight ~55 kDa.

Western blot testing of 1) rat brain, 2) mouse brain and 3) mouse NIH 3T3 lysate wtih SLC2A1 antibody. Predicted molecular weight ~55 kDa.

Flow cytometry testing of human A431 cells with SLC2A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SLC2A1 antibody.