SLC22A8 Antibody / OAT3 (RQ7661)

SLC22A8 / OAT3 Antibody Prostate Cancer IF. Immunofluorescence staining of FFPE human prostate cancer tissue using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates distinct green fluorescence within subsets of epithelial tumor-associated cells, consistent with expression of the membrane transporter SLC22A8/OAT3. Signal is observed in discrete cellular populations against a background of DAPI-counterstained nuclei (blue), supporting evaluation of transporter expression and localization in human prostate cancer tissue. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining. Target protein is visualized in green and nuclei are shown in blue.

SLC22A8 / OAT3 Antibody Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates moderate to strong HRP-DAB brown staining within malignant epithelial cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of SLC22A8/OAT3, a transmembrane organic anion transporter involved in cellular uptake and transport of endogenous metabolites and xenobiotic compounds. These results support evaluation of OAT3 expression and localization in human breast cancer tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

SLC22A8 / OAT3 Antibody Prostate Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human prostate adenocarcinoma tissue using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates moderate to strong HRP-DAB brown staining within malignant glandular epithelial cells. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of SLC22A8/OAT3, a transmembrane transporter involved in organic anion uptake and cellular transport of endogenous metabolites and xenobiotic compounds. Staining highlights neoplastic glandular structures within the tumor microenvironment and supports evaluation of OAT3 expression and localization in human prostate adenocarcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

SLC22A8 / OAT3 Antibody Larynx Squamous Cell Carcinoma IHC. Immunohistochemistry staining of FFPE human larynx squamous cell carcinoma tissue using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates moderate to strong HRP-DAB brown staining within malignant squamous epithelial cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of SLC22A8/OAT3, a transmembrane organic anion transporter involved in cellular uptake and transport of endogenous metabolites, signaling molecules, and xenobiotic compounds. Staining highlights tumor-associated epithelial nests and supports evaluation of OAT3 expression and localization in human laryngeal squamous cell carcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

SLC22A8 / OAT3 Antibody Liver Cancer IHC. Immunohistochemistry staining of FFPE human liver cancer tissue using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates moderate to strong HRP-DAB brown staining within malignant hepatocyte-like tumor cell populations. The observed staining pattern is predominantly membranous and cytoplasmic, consistent with expression of SLC22A8/OAT3, a transporter involved in organic anion uptake and cellular handling of endogenous metabolites, drugs, and xenobiotic compounds. Staining highlights tumor-associated epithelial cells and supports evaluation of OAT3 expression and localization in human liver cancer tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining.

SLC22A8 / OAT3 Antibody Human 293T WB. Western blot analysis of human 293T cell lysate using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates a distinct band at approximately 60 kDa, consistent with the predicted molecular weight of SLC22A8/OAT3. OAT3 is a membrane-associated organic anion transporter involved in the cellular uptake and transport of endogenous metabolites, pharmaceutical compounds, and xenobiotic substances. The observed band supports detection of OAT3 expression in human cells and validates the antibody for Western blot applications.

SLC22A8 / OAT3 Antibody K562 FACS. Flow cytometry analysis of human K562 cells using SLC22A8 Antibody / Organic Anion Transporter 3 (OAT3) Antibody demonstrates a clear positive fluorescence shift relative to both unstained cells and isotype control-treated cells, consistent with expression of SLC22A8/OAT3. Overlay histograms compare cells alone (red), isotype control antibody (green), and SLC22A8/OAT3 antibody (blue), with the specific antibody producing a marked rightward shift indicative of target-associated binding. Cells were blocked with goat serum prior to staining, and antibody was used at 1 μg per million cells. These results support application of the antibody for flow cytometric detection of OAT3 expression in human cell populations.