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Home >> Antibodies >> SIX4 Antibody / Sine oculis homeobox homolog 4

SIX4 Antibody / Sine oculis homeobox homolog 4 (RQ8216)

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  Catalog No Formulation Size Price (USD)  
Image RQ8216 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human U-2 OS cells with SIX4 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with SIX4 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A431, 2) RT4 and 3) U-2 OS cell lysate with SIX4 antibody. Predicted molecular weight ~83 kDa, commonly observed at 83-98 kDa.
Flow cytometry testing of fixed and permeabilized human U937 cells with SIX4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIX4 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UIU6
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SIX4 antibody is available for research use only.
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Description

Homeobox protein SIX4 is a protein that in humans is encoded by the SIX4 gene. This gene encodes a member of the homeobox family, subfamily SIX. The drosophila homolog is a nuclear homeoprotein required for eye development. Studies in mouse show that this gene product functions as a transcription factor, and may have a role in the differentiation or maturation of neuronal cells.

Application Notes

Optimal dilution of the SIX4 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q387-L781) was used as the immunogen for the SIX4 antibody.

Storage

After reconstitution, the SIX4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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