Sirtuin 5 Antibody / Mitochondrial Deacylase Antibody (RQ7422)

Sirtuin 5 Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue demonstrated moderate to strong Sirtuin 5 expression with predominantly cytoplasmic staining in malignant glandular epithelial cells and weaker staining in the surrounding stroma. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The staining pattern is consistent with the predominantly mitochondrial localization of this Mitochondrial Deacylase, which regulates protein deacylation and cellular metabolism. This Sirtuin 5 Antibody, a Mitochondrial Deacylase Antibody, is suitable for immunohistochemical studies of mitochondrial metabolism, protein acylation and cancer biology.

Sirtuin 5 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemical staining of FFPE human colorectal adenocarcinoma tissue demonstrated strong Sirtuin 5 expression with predominantly cytoplasmic staining in malignant glandular epithelial cells. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The staining pattern is consistent with the predominantly mitochondrial localization of this Mitochondrial Deacylase, which regulates lysine deacylation, mitochondrial metabolism and cellular energy homeostasis. This Sirtuin 5 Antibody, a Mitochondrial Deacylase Antibody, is suitable for immunohistochemical studies of metabolic regulation, mitochondrial function and cancer biology.

Sirtuin 5 Antibody Rat Colon IHC. Immunohistochemical staining of FFPE rat colon demonstrated moderate to strong Sirtuin 5 expression with predominantly cytoplasmic staining in the epithelial cells lining the colonic glands. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The staining pattern is consistent with the predominantly mitochondrial localization of this Mitochondrial Deacylase, which regulates lysine deacylation, energy metabolism and mitochondrial protein function. This Sirtuin 5 Antibody, a Mitochondrial Deacylase Antibody, is suitable for immunohistochemical studies of mitochondrial metabolism, intestinal physiology and metabolic regulation.

Sirtuin 5 Antibody Human, Mouse and Rat WB. Western blot analysis detected Sirtuin 5 in human hepatocellular carcinoma tissue (lane 1), human hepatocellular carcinoma cells (lane 2), human HepG2 cells (lane 3), human 293T cells (lane 4), rat liver (lane 5) and mouse liver (lane 6). A prominent immunoreactive band was observed at approximately 32–33 kDa, consistent with the expected molecular weight of mature Sirtuin 5 following mitochondrial targeting sequence processing. This Sirtuin 5 Antibody, a Mitochondrial Deacylase Antibody, demonstrates cross-species reactivity and is suitable for studies of mitochondrial metabolism, lysine deacylation and cellular energy homeostasis.

Sirtuin 5 Antibody Daudi Cell FACS. Flow cytometry analysis of fixed and permeabilized Daudi cells demonstrated a clear rightward fluorescence shift with Sirtuin 5 Antibody (blue) compared with the isotype control (green) and unstained cells (red), indicating specific detection of intracellular Sirtuin 5 expression. Cells were stained with 1 ug antibody per 1×10^6 cells followed by a Fluoro488-conjugated secondary antibody. This Sirtuin 5 Antibody, a Mitochondrial Deacylase Antibody, is suitable for flow cytometric analysis of intracellular protein expression in studies of mitochondrial metabolism, lysine deacylation and cellular energy homeostasis.