SIRT3 Antibody / Mitochondrial Metabolism Regulator [clone PCRP-SIRT3-1C10] (V9646)

SIRT3 Antibody HeLa Cell IF. Immunofluorescence staining of PFA-fixed human HeLa cells using clone PCRP-SIRT3-1C10 (green) and phalloidin cytoskeletal counterstain (red). Sirtuin 3 / SIRT3 displays punctate cytoplasmic fluorescence consistent with mitochondrial localization, supporting its established role as a mitochondrial metabolism regulator involved in oxidative stress adaptation, energy homeostasis, and mitochondrial acetylation signaling pathways.

SIRT3 Antibody HeLa Cell FACS. Flow cytometry analysis of PFA-fixed human HeLa cells stained with clone PCRP-SIRT3-1C10 (blue) and matched isotype control (red). The observed fluorescence shift demonstrates cellular expression of Sirtuin 3 / SIRT3, a mitochondrial deacetylase involved in regulation of oxidative metabolism, mitochondrial homeostasis, and metabolic stress response pathways associated with cellular energy adaptation.

SDS-PAGE analysis of purified, BSA-free SIRT3 antibody (clone PCRP-SIRT3-1C10) as confirmation of integrity and purity.

SIRT3 Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone PCRP-SIRT3-1C10. These results demonstrate high specificity of the mouse monoclonal antibody for Sirtuin 3 / SIRT3, a mitochondrial metabolism regulator involved in oxidative stress adaptation, energy homeostasis, and acetylation-dependent metabolic signaling pathways. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.