SIRT1 Antibody (RQ5549)

IHC staining of FFPE human intestinal cancer with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE mouse testis with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat testis with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Western blot testing of 1) rat testis, 2) mouse thymus, 3) mouse testis, 4) mouse NIH3T3 and 5) human HL-60 lysate with SIRT1 antibody. Predicted molecular weight ~80 kDa but is routinely observed at 110~120 kDa due to post-translational modification.

Flow cytometry testing of human HeLa cells with SIRT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIRT1 antibody.