SIRT1 Antibody / Metabolic Stress Regulator [clone PCRP-SIRT1-1E11] (V9647)

SIRT1 Antibody HeLa Cell IF. Immunofluorescence staining of PFA-fixed human HeLa cells using clone PCRP-SIRT1-1E11 (green) and phalloidin cytoskeletal counterstain (red). Sirtuin 1 / SIRT1 displays predominantly nuclear and cytoplasmic fluorescence consistent with its role as a metabolic stress regulator involved in acetylation-dependent transcriptional control, chromatin signaling, and cellular adaptation to oxidative and nutrient stress pathways.

SIRT1 Antibody HeLa Cell FACS. Flow cytometry analysis of PFA-fixed human HeLa cells stained with clone PCRP-SIRT1-1E11 (blue) and matched isotype control (red). The observed fluorescence shift demonstrates cellular expression of Sirtuin 1 / SIRT1, a NAD-dependent deacetylase involved in metabolic stress signaling, chromatin regulation, and adaptive cellular responses to oxidative and nutrient stress conditions.

SDS-PAGE analysis of purified, BSA-free SIRT1 Antibody / Metabolic Stress Regulator (clone PCRP-SIRT1-1E11) as confirmation of integrity and purity.

SIRT1 Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone PCRP-SIRT1-1E11. These results demonstrate selective recognition of Sirtuin 1 / SIRT1, a metabolic stress regulator involved in NAD-dependent deacetylation, chromatin signaling, and adaptive cellular stress-response pathways. Z- and S-score analysis supports preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.