- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
SIPA1 Antibody / Signal-induced proliferation-associated 1 (FY12879)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12879 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
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SIPA1 antibody detects Signal-induced proliferation-associated 1, a Rap GTPase-activating protein (GAP) involved in cell adhesion, migration, and cell cycle progression. Encoded by the SIPA1 gene on chromosome 11q13.1, this signaling molecule terminates Ras-related protein Rap1 and Rap2 signaling by accelerating GTP hydrolysis, thereby modulating integrin-mediated adhesion and mitogenic responses. SIPA1 plays a crucial role in immune cell trafficking, vascular remodeling, and tumor metastasis suppression.
Structurally, SIPA1 contains an N-terminal RapGAP domain responsible for GTPase activation, a PDZ domain that mediates protein-protein interactions, and a C-terminal coiled-coil domain that regulates subcellular localization. It is predominantly localized to the cytoplasm and nucleus, where it integrates extracellular growth and adhesion cues with downstream transcriptional and cytoskeletal responses. SIPA1 interacts with integrins and focal adhesion components, influencing adhesion turnover and migration.
The SIPA1 antibody is widely used in cell signaling, oncology, and vascular biology research to study Rap GTPase regulation, adhesion signaling, and cancer cell dissemination. Western blot analysis detects a 120 kilodalton band corresponding to SIPA1, while immunofluorescence shows cytoplasmic and nuclear staining in epithelial and immune cells. This antibody enables characterization of Rap1 deactivation and adhesion dynamics under physiological and pathological conditions.
Functionally, SIPA1 acts as a metastasis suppressor by downregulating integrin signaling and preventing excessive adhesion and proliferation. Reduced expression or loss of SIPA1 disrupts Rap1 regulation, leading to enhanced cell spreading, migration, and metastasis formation in several cancers. In immune cells, SIPA1 modulates adhesion to vascular endothelium and migration across tissue barriers, linking Rap signaling to immune surveillance and inflammation. The SIPA1 antibody supports studies aimed at understanding how RapGAP activity integrates with cytoskeletal and transcriptional control mechanisms. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring high-quality and consistent performance for signaling and adhesion studies.
Optimal dilution of the SIPA1 antibody should be determined by the researcher.
E.coli-derived human SIPA1 recombinant protein (Position: D21-L1034) was used as the immunogen for the SIPA1 antibody.
After reconstitution, the SIPA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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