SIGLEC-4a Antibody / Sialic Acid Binding Immunoglobulin Like Lectin 4A Antibody [clone 2G11] (RQ6281)

SIGLEC-4a Antibody Rat Cerebellum IF. Immunofluorescent staining of FFPE rat cerebellum tissue using SIGLEC-4a antibody (green) with GFAP antibody (red) and DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed in pH6 citrate buffer, followed by incubation with the primary antibodies and fluorescent secondary antibodies. SIGLEC-4a immunoreactivity is prominently localized within the cerebellar white matter, consistent with expression in myelinating glial cells, while GFAP labels astrocytes. This result demonstrates that SIGLEC-4a antibody, also known as a Sialic Acid Binding Immunoglobulin Like Lectin 4A antibody, is suitable for immunofluorescence analysis of rat nervous tissue.

SIGLEC-4a Antibody Rat Brain IHC. IHC staining of FFPE rat brain tissue using SIGLEC-4a antibody. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before incubation with the primary antibody, followed by HRP secondary antibody and DAB chromogen. Strong immunoreactivity is observed within myelinated white matter tracts, while adjacent gray matter exhibits comparatively weaker staining, consistent with the distribution of myelin-associated glycoprotein in the central nervous system. This result demonstrates that SIGLEC-4a antibody, also known as a Sialic Acid Binding Immunoglobulin Like Lectin 4A antibody, is suitable for immunohistochemical detection of myelin-associated structures in rat brain tissue.

SIGLEC-4a Antibody Mouse Brain IHC. IHC staining of FFPE mouse brain tissue using SIGLEC-4a antibody. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before incubation with the primary antibody, followed by HRP secondary antibody and DAB chromogen. Diffuse membranous and cytoplasmic immunoreactivity is observed throughout myelin-rich regions of the brain, consistent with the expression pattern of myelin associated glycoprotein in oligodendrocytes and myelinated fibers. This result demonstrates that SIGLEC-4a antibody, also known as a Sialic Acid Binding Immunoglobulin Like Lectin 4A antibody, is suitable for immunohistochemical detection of myelin-associated proteins in mouse brain tissue.

SIGLEC-4a Antibody Rat and Mouse Brain WB. Western blot analysis of rat and mouse brain lysates using SIGLEC-4a antibody. Lane 1: rat brain. Lane 2: mouse brain. A broad, intense band cluster is detected between approximately 90-130 kDa in both species, consistent with the highly glycosylated forms of myelin associated glycoprotein (MAG) found in central nervous system tissue. Additional bands at and just below approximately 72 kDa likely represent lower-molecular-weight MAG isoforms and/or less-glycosylated variants. The observed migration above the predicted molecular weight of approximately 69 kDa is expected due to extensive glycosylation and glycoform heterogeneity. These results demonstrate that SIGLEC-4a antibody, also known as a Sialic Acid Binding Immunoglobulin Like Lectin 4A antibody, reliably detects endogenous MAG in rodent brain by Western blot.

SIGLEC-4a Antibody Human Glioblastoma Cell FACS. Flow cytometry analysis of fixed and permeabilized human U-87 MG glioblastoma cells using SIGLEC-4a antibody at 1 ug/10^6 cells following blocking with goat serum. Red: cells alone. Green: isotype control. Blue: SIGLEC-4a antibody. A clear rightward shift of the antibody-stained population relative to the isotype control demonstrates specific detection of endogenous SIGLEC-4a expression in U-87 MG cells. This result shows that SIGLEC-4a antibody, also known as a Sialic Acid Binding Immunoglobulin Like Lectin 4A antibody, is suitable for flow cytometric analysis of human glioblastoma cells.