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Home >> Antibodies >> SHMT Antibody / SHMT1

SHMT Antibody / SHMT1 [clone 9C7] (RQ6407)

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  Catalog No Formulation Size Price (USD)  
Image RQ6407 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human liver cancer tissue with SHMT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with SHMT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with SHMT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) monkey liver, 2) rat liver and 3) mouse liver tissue lysate with SHMT antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human A431 cells with SHMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SHMT antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 9C7
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P34896
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This SHMT antibody is available for research use only.
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  • Applications : WB, IF, FACS, Direct ELISA
    Reactivity : Human, Mouse, Rat, Monkey

Description

This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the SHMT antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-S470) was used as the immunogen for the SHMT antibody.

Storage

After reconstitution, the SHMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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