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Home >> Antibodies >> SH2D4A Antibody / PPP1R38

SH2D4A Antibody / PPP1R38 (RQ7645)

  Catalog No Formulation Size Price (USD)  
Image RQ7645 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human liver cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat gastric tissue with SH2D4A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with SH2D4A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HeLa and 3) rat PC-12 cell lysate with SH2D4A antibody. Predicted molecular weight: ~53 kDa.
Flow cytometry testing of human A549 cells with SH2D4A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SH2D4A antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H788
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SH2D4A antibody is available for research use only.
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Description

SH2 domain-containing protein 4A, also called Protein phosphatase 1 regulatory subunit 38 (PPP1R38) is a protein that in humans is encoded by the SH2D4A gene. Using exon trapping and exon linking at chromosome 8p22 cloned SH2D4A, which they called SH2A. The deduced 454-amino acid protein contains a single SH2 domain. RT-PCR and Northern blot analysis detected ubiquitous expression of 3 SH2A transcripts, as well as aberrant expression in some cancer cell lines. Dai et al. (2002) concluded that SH2A is a docking protein that may be involved in signal transduction.

Application Notes

Optimal dilution of the SH2D4A antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R42-E454) was used as the immunogen for the SH2D4A antibody.

Storage

After reconstitution, the SH2D4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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