SFMBT2 Antibody / Scm-like with four MBT domains protein 2 (FY13006)

Immunofluorescent staining of SFMBT2 using anti-SFMBT2 antibody (green) and anti-Beta Tubulin antibody (red). SFMBT2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SFMBT2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of SFMBT2 using anti-SFMBT2 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFMBT2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for SFMBT2 at approximately 101 kDa. The expected molecular weight of SFMBT2 is ~101 kDa.

Immunoprecipitating SFMBT2 in Jurkat whole cell lysate. Western blot analysis of SFMBT2 using anti-SFMBT2 antibody. Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SFMBT2 antibody in Jurkat whole cell lysate, Lane 3: anti-SFMBT2 antibody (2ug) + Jurkat whole cell lysate (500ug). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFMBT2 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SFMBT2 at approximately 101 kDa. The expected molecular weight of SFMBT2 is at 101 kDa.