SF3a66 Antibody / Splicing factor 3A subunit 2 / SF3A2 (RQ8173)

IHC staining of FFPE human testicular seminoma tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placenta tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human urothelial carcinoma tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human rectum adenocarcinoma tissue with SF3a66 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with SF3a66 antibody (green) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A549, 4) human SH-SY5Y, 5) rat thymus, 6) rat ovary, 7) mouse thymus and 8) mouse ovary tissue lysate with SF3a66 antibody. Predicted molecular weight ~49 kDa but commonly observed at ~66 kDa.

Flow cytometry testing of fixed and permeabilized human SiHa cells with SF3a66 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SF3a66 antibody.