- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
SF3a60 Antibody / SAP 61 / Splicing factor 3A subunit 3 (RQ7634)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7634 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q12874 |
| Localization | Nuclear |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Immunoprecipitation : 2ug per 500ug of lysate Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This SF3a60 antibody is available for research use only. |
|
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SF3a60 antibody detects Splicing factor 3A subunit 3, also known as SAP 61 or SF3A3, a core component of the essential SF3A spliceosomal complex. The UniProt recommended name is Splicing factor 3A subunit 3 (SF3A3). As one of the three SF3A subunits (SF3A1, SF3A2, and SF3A3), this protein is required for stable incorporation of the U2 small nuclear ribonucleoprotein (U2 snRNP) into the pre-spliceosomal A complex. This step is fundamental to early spliceosome assembly and ensures accurate recognition of the branch point sequence during pre-mRNA splicing.
Functionally, SF3a60 antibody identifies a protein of approximately 239 amino acids that localizes to the nucleus, particularly within splicing factorârich speckles associated with active transcription. SF3A3 forms part of the SF3A heterotrimer by interacting directly with SF3A1 and SF3A2, providing structural support that stabilizes U2 snRNP engagement with the pre-mRNA substrate. SF3A3 contributes to RNA-binding and proteinâprotein interactions necessary for configuring the A complex in a way that correctly positions the branch point adenosine for subsequent spliceosome remodeling. Because of this positioning role, SF3A3 is indispensable for accurate intron removal and splice site fidelity.
The SF3A3 gene is located on chromosome 1q21.2 and is expressed in all mammalian tissues, reflecting its universal requirement for mRNA maturation. Expression is particularly prominent in rapidly dividing and transcriptionally active cells, including hematopoietic progenitors, gastrointestinal epithelium, and various tumors. Within the nucleus, SF3A3 dynamically redistributes in response to transcriptional activity, cellular stress, and signaling cues that influence alternative splicing patterns. Its interactions extend beyond the SF3A complex to proteins associated with chromatin modulation, transcriptional elongation, and RNA surveillance, highlighting its integrative role in coordinating gene expression and RNA quality control.
SF3A3 plays a functional role not only in constitutive splicing but also in alternative splicing decisions that regulate developmental pathways, neuronal identity, immune responses, and metabolic adaptation. Perturbation of SF3A3 levels or interactions can lead to widespread splicing defects, including exon skipping, intron retention, and production of aberrant mRNA isoforms. These alterations can influence protein stability, signaling pathway output, and cellular homeostasis. Experimental studies suggest that SF3A3 helps maintain the fidelity and efficiency of the splicing machinery, especially under conditions of high transcriptional load or stress.
Altered expression of SF3A3 has been implicated in cancer progression, neurodegenerative disease, and immune dysfunction. In several malignancies, elevated SF3A3 expression correlates with increased proliferative capacity and global shifts in splicing that favor oncogenic pathways. Tumors with spliceosome dysregulation often show dependency on SF3A components, including SF3A3, for survival, making this complex a potential therapeutic target. Conversely, reduced function of spliceosomal proteins, including SF3A3, may contribute to impaired RNA metabolism observed in aging-related neuronal decline and in certain genetic disorders involving RNA-binding protein dysfunction. Because RNA splicing is intimately linked with gene expression plasticity, disruptions in SF3A3-mediated processes can have far-reaching effects on cell health and phenotype.
Research using SF3a60 antibody supports investigations into spliceosome assembly, nuclear speckle organization, alternative splicing patterns, transcription-splicing coupling, and the molecular underpinnings of diseases involving RNA processing defects. SF3a60 antibody is validated for use in relevant research applications to detect Splicing factor 3A subunit 3 expression and examine its roles in nuclear RNA metabolism and gene regulation. NSJ Bioreagents provides SF3a60 antibody reagents optimized for studies in RNA biology, developmental regulation, cancer research, and transcriptome integrity.
Optimal dilution of the SF3a60 antibody should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids E123-Q472) was used as the immunogen for the SF3a60 antibody.
After reconstitution, the SF3a60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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