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Home >> Antibodies >> SF3a120 Antibody / Splicing factor 3A subunit 1 / SF3A1

SF3a120 Antibody / Splicing factor 3A subunit 1 / SF3A1 (RQ7633)

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  Catalog No Formulation Size Price (USD)  
Image RQ7633 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat lung, 2) rat brain, 3) rat thymus, 4) rat C6, 5) mouse lung, 6) mouse brain, 7) mouse thymus and 8) mouse Neuro-2a cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
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Western blot testing of 1) rat lung, 2) rat brain, 3) rat thymus, 4) rat C6, 5) mouse lung, 6) mouse brain, 7) mouse thymus and 8) mouse Neuro-2a cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) 293T, 5) U-251, 6) Daudi and 7) MOLT4 cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.
IHC staining of FFPE human ovarian serous cancer tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human larynx squamous cell carcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with SF3a120 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human intestinal cancer tissue with SF3a120 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat colon tissue with SF3a120 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with SF3a120 antibody (red) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q15459
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This SF3a120 antibody is available for research use only.
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Description

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene. This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing.

Application Notes

Optimal dilution of the SF3a120 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids K20-Q556) was used as the immunogen for the SF3a120 antibody.

Storage

After reconstitution, the SF3a120 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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